Cell Signaling Technology

Product Pathways - NF-kB Signaling

Phospho-IRF-3 (Ser396) (4D4G) Rabbit mAb #4947

interferon regulatory factor   IRF  

No. Size Price
4947S 100 µl ( 10 western blots ) ¥3,780.00 现货查询 购买询价
4947 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 45-55 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Monkey, Pig,

Specificity / Sensitivity

Phospho-IRF-3 (Ser396) (4D4G) Rabbit mAb detects endogenous levels of IRF-3 when phosphorylated at Ser396.

Phospho-IRF-3 (Ser396) (4D4G) Rabbit mAb兔单抗只能检测内源的在Ser396位点磷酸化的IRF-3蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser396 of human IRF-3.

此单克隆抗体是通过合成人源对应的IRF-3 Ser396位点周围的磷肽段来免疫动物而获得。

Western Blotting

Western Blotting

Western blot analysis of extracts from HT29 and THP1 cells, control or plpC-transfected (1 hour), using Phospho-IRF-3 (Ser396) (4D4G) Rabbit mAb.Western免疫印迹。用Phospho-IRF-3 (Ser396) (4D4G) Rabbit mAb研究对照和plpC转染1小时的 HT29和THP1细胞的细胞提取液。


Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, ISGF3γ/p48, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS) and IFN regulatory elements (IRF-E) (2).

干扰素调控因子(IRFs) 包含一个家族的转录调控因子,这个家族主要是在 Jak/Stat信号通路中起作用,在受到病毒的感染后能够调控干扰素(IFN)和干扰素诱导的基因表达(1)。IRFs在抵抗病原菌、自身免疫、淋巴细胞发育、细胞生长和易感性的转变等过程中发挥了重要的作用。IRF家族包含9个成员: IRF-1, IRF-2, ISGF3γ/p48, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7和IRF-8/ICSBP。所有的IRF蛋白在N-末端的DNA-结合区域都有同源性。IRF家族成员通过与具有相似的DNA-结合区域的蛋白的反应而调控转录,例如IFN刺激的应答原件(ISRE), IFN 保守基序 (ICS) 和IFN 调控元件(IRF-E) (2)。

IRF-3 can inhibit cell growth and plays a critical role in controlling the expression of genes in the innate immune response (1-4). In unstimulated cells, IRF-3 is present in the cytoplasm. Viral infection results in phosphorylation of IRF-3 and leads to its translocation to the nucleus where it activates promoters containing IRF-3-binding sites. Phosphorylation of IRF-3 occurs at a cluster of C-terminal serine and threonine residues (between 385 and 405) leading to its association with the p300/CBP coactivator protein that promotes DNA binding and transcriptional activity (5). During infection, IRF-3 is likely activated through a pathway that includes activation of Toll-like receptors and of a kinase complex that includes IKKε and TBK1 (6,7). IRF-3 is phosphorylated at Ser396 following viral infection, expression of viral nucleocapsid, and double stranded RNA treatment. These events likely play a role in activation of IRF-3 (8).

IRF-3能抑制细胞的生长并在调控天然免疫应答基因中发挥了重要的作用(1-4)。在未受到刺激的细胞中, IRF-3 存在于细胞质中。病毒感染导致IRF-3磷酸化及其在细胞核的定位并激活具有IRF-3结合位点的启动子。IRF-3的磷酸化位点位于C-末端丝氨酸和苏氨酸簇中 (在385和405位点之间)导致了其与p300/CBP共激活蛋白的结合,从而促进DNA的结合和转录的活性(5)。在感染期间, IRF-3很可能是通过包括TLR和IKKε和 TBK1激酶复合体在内的一系列的信号通路而激活(6,7)。在受到病毒的感染、病毒核蛋白壳表达以及双链RNA的处理后,IRF-3在Ser396位点发生磷酸化,这些事件对IRF-3的激活非常重要(8)。

  1. Taniguchi, T. et al. (2001) Annu Rev Immunol 19, 623-55.
  2. Honda, K. and Taniguchi, T. (2006) Nat Rev Immunol 6, 644-58.
  3. Hiscott, J. et al. (1999) J. Interferon Cytokine Res. 19, 1-13.
  4. Kim, T.Y. et al. (2003) J. Biol. Chem. 278, 15272-15278.
  5. Yoneyama, M. et al. (2002) J. Interferon Cytokine Res. 22, 73-76.
  6. Fitzgerald, K.A. et al. (2003) Nat. Immunol. 4, 491-496.
  7. Kopp, E. and Medzhitov, R. (2003) Curr. Opin. Immunol. 15, 396-401.
  8. Servant, M.J. et al. (2003) J. Biol. Chem. 278, 9441-9447.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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