Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

Phospho-WNK1 (Thr60) Antibody #4946

No. Size Price
4946S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
4946 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 230 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Mouse, Rat,

Specificity / Sensitivity

Phospho-WNK1 (Thr60) Antibody detects endogenous levels of WNK1 only when phosphorylated at threonine 60.

Phospho-WNK1 (Thr60) Antibody可以检测在Thr60位点磷酸化的内源性总WNK1蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresonding a region surrounding Thr60 of human WNK1. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from HT29 cells, untreated or IGF1-treated (100 ng/ml for 30 minutes), using Phospho-WNK1 (Thr60) Antibody (upper) or WNK1 Antibody #4979 (lower).

Weatern blot 分析HT29细胞提取物,未刺激组和IGF1 (100 ng/ml ,30 分钟)处理组,所用抗体为Phospho-WNK1 (Thr60) Antibody (上)和WNK1 Antibody #4979 (下).


The WNK [with no lysine (K)] family of serine/threonine kinases is characterized by having a cysteine in place of lysine in subdomain II of its kinase activation domain (1,2). The lysine necessary for phosphoryl transfer is located in an atypical position in the catalytic domain. Four WNK family members have been identified in humans (WNK1-4) and have been implicated in regulating ion permeability (3). Mutations in the WNK1 and WNK4 genes in humans cause pseudohypoaldosteronism type II (PHAII), an autosomal dominant disorder leading to hypertension, hyperkalemia, and renal tubular acidosis (4). WNK4 is specifically expressed in the kidney, whereas WNK1 has a wider distribution but is predominantly expressed in polarized epithelia (1-3). Heterozygous mutations in WNK1 in mice result in a significant decrease in blood pressure, while homozygous mutations are embryonic lethal (5). WNK1 is phosphorylated by Akt at Thr60 (6). In addition, WNK1 may be autophosphorylated at Ser382 in the activation loop, and this is thought to be required for its kinase activity (7).

WNK[无赖氨酸(K)]丝氨酸和苏氨酸激酶家族的特点是其激酶活性域的子域II 赖氨酸被半胱氨酸取代(1、2)。赖氨酸对于磷酰基转移时必需的,在催化域的非典型位置。4个WNK家族成员已经在人类被确认(WNK1-4),参与离子通透(3)。人类WNK1 和 WNK4基因的突变可以引起假性醛固酮减少症类型II (PHAII), 是常染色体显性遗传病,可以引起高血压,高钾血症和肾小管硬化(4), WNK4特异性表达在肾脏,而WNK1则广泛表达,主要表达在极化的上皮细胞上(1-3)。小鼠WNK1的杂合突变引起血压的显著降低,然而纯合突变会引起胚胎死亡(5). WNK1在Thr60位点被Akt磷酸化(6)。另外, WNK1 在活化畔中在Ser382位点发生自磷酸化,被认为是激酶活性必须(7)。

  1. Veríssimo, F. and Jordan, P. (2001) Oncogene 20, 5562-9.
  2. Xu, B. et al. (2000) J Biol Chem 275, 16795-801.
  3. Choate, K.A. et al. (2003) Proc Natl Acad Sci U S A 100, 663-8.
  4. Wilson, F.H. et al. (2001) Science 293, 1107-12.
  5. Zambrowicz, B.P. et al. (2003) Proc Natl Acad Sci U S A 100, 14109-14.
  6. Vitari, A.C. et al. (2004) Biochem J 378, 257-68.
  7. Xu, B.E. et al. (2002) J Biol Chem 277, 48456-62.

Application References

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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