Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

CENP-I (D4D5K) Rabbit mAb #49426

No. Size Price
49426S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
49426 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 80 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

CENP-I (D4D5K) Rabbit mAb recognizes endogenous levels of total CENP-I protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn41 of human CENP-I protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using CENP-I (D4D5K) Rabbit mAb.

Background

Kinetochores are mitotic structures that form on centromeres and attach to mitotic spindle microtubules. Kinetochore attachment to microtubules regulates chromosome segregation and progression through mitosis. Unattached kinetochores signal to the spindle assembly checkpoint (SAC) machinery, arresting cells in mitosis (1).

CENP-I is a centromere protein associated with the inner kinetochore, and required for progression through mitosis and the appropriate localization of other kinetochore-associated and checkpoint proteins, including CENP-C, the RZZ complex, MAD1 and MAD2 (2-4).

  1. Foley, E.A. and Kapoor, T.M. (2013) Nat Rev Mol Cell Biol 14, 25-37.
  2. Nishihashi, A. et al. (2002) Dev Cell 2, 463-76.
  3. Matson, D.R. and Stukenberg, P.T. (2014) J Cell Biol 205, 541-54.
  4. Liu, S.T. et al. (2003) Nat Cell Biol 5, 341-5.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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