Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Phospho-PBK/TOPK (Thr9) Antibody #4941

PBK   PDZ-binding kinase   T-LAK cell-originated protein kinase   TOPK  

No. Size Price
4941S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
4941T 20 µl ( 2 western blots ) ¥1,300.00 现货查询 购买询价
4941 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 40 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-PBK/TOPK (Thr9) Antibody detects endogenous levels of PBK/TOPK only when phosphorylated at threonine 9.Phospho-PBK/TOPK (Thr9) Antibody能够检测内源性苏氨酸(9位)磷酸化的PBK/TOPK 蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to amino acids around Thr9 of human PBK/TOPK. Antibodies are purified by protein A and affinity chromatography.该多克隆抗体是由合成的人源的针对PBK/TOPK 蛋白苏氨酸(9位)的磷酸化肽段免疫动物,采用蛋白A和多肽亲和层析技术纯化生产的。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of untreated Jurkat cells, using Phospho-PBK/TOPK (Thr9) Antibody versus propidium iodide (DNA content). The boxed population indicates phospho-PBK/TOPK (Thr9)-positive cells.流式细胞术检测未处理的Jurkat细胞,使用的抗体为Phospho-PBK/TOPK (Thr9) Antibody ,碘化乙啶检测DNA含量作为对照。框内的细胞为磷酸化PBK/TOPK (Thr9)阳性的细胞。

Western Blotting

Western Blotting

Western blot analysis of extracts from HT29 cells, untreated or nocodazole-treated (50ng/ml), using Phospho-PBK/TOPK (Thr9) Antibody (upper) or PBK/TOPK Antibody #4942 (lower).Western blot 方法检测HT29细胞提取物,分为未处理组和诺考达唑(50ng/ml)处理组,使用的抗体为 Phospho-PBK/TOPK (Thr9) Antibody (上图) 或 PBK/TOPK Antibody #4942 (下图)。

IF-IC

IF-IC

Confocal immunofluorescent analysis of HT-29 cells, untreated (left) or λ phosphatase-treated (right), using Phospho-PBK/TOPK (Thr9) Antibody (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).激光共聚焦免疫荧光方法检测HT-29细胞,分为非处理组(左图)和λ 磷酸酶处理组(右图),使用的抗体为Phospho-PBK/TOPK (Thr9) Antibody ,呈绿色。肌动蛋白纤维由DY-554鬼笔环肽标记,呈红色。蓝色伪彩为DNA荧光染料,产品信息DRAQ5® #4084。

Background

PBK/TOPK is a serine/threonine kinase that is phosphorylated and active during mitosis (1). PBK/TOPK is composed of kinase subdomains and a carboxy-terminal PDZ-Binding domain, which is thought to interact with the tumor suppressor protein hDlg (1). Increased PBK/TOPK expression has been observed in highly proliferative malignant cell lines, and PBK/TOPK expression is strongly downregulated during terminal differentiation of HL-60 leukemic cells (2,3). PMA-induced kinase activity toward PBK/TOPK has been observed (4), and cdc2/cyclinB has been shown to phosphorylate PBK/TOPK in vitro, presumably at Thr9 (1). Potential substrates of PBK/TOPK include p38 MAPK and c-Myc (3,4). PBK/TOPK是一种丝氨酸/苏氨酸激酶,在有丝分裂过程中被磷酸化和活化(1)。PBK/TOPK由激酶亚结构域和一个羧基末端PDZ-结合结构域,该结构域被认为能够和肿瘤抑制蛋白hDlg相互作用(1)。在高度增殖的恶性肿瘤细胞系中已观察到PBK/TOPK的表达增加,而在终端分化的HL-60白血病细胞中观察到PBK/TOPK的显著下调(2,3)。PMA-诱导的针对PBK/TOPK的激酶活性也被观察到,且cdc2/cyclinB被认为可以离体磷酸化PBK/TOPK,可能的位点是第9位苏氨酸(1)。PBK/TOPK的潜在底物包括p38 MAPK 和c-Myc (3,4)。

  1. Gaudet, S. et al. (2000) Proc. Natl. Acad. Sci. U S A 97, 5167-5172.
  2. Simons-Evelyn, M. et al. (2001) Blood Cells Mol. Dis. 27, 825-829.
  3. Nandi, A. et al. (2004) Blood Cells Mol. Dis. 32, 240-245.
  4. Abe, Y. et al. (2000) J. Biol. Chem. 276, 21525-21531.
  5. Matsumoto, S. et al. (2004) Biochem Biophys Res Commun. 325, 997-1004.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications