Cell Signaling Technology

Product Pathways - Protein Stability

SUMO-1 (C9H1) Rabbit mAb #4940

ubiquitin  

No. Size Price
4940S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
4940 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous Rabbit IgG
IP 1:50
IHC-P 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

SUMO-1 (C9H1) 兔单抗识别重组的SUMO-1蛋白和内源性的SUMO化蛋白(如SUMO-1-RanGAP,90kD),这一抗体不能用于重组SUMO-2或SUMO-3的检测。

Source / Purification

此单抗经合成与人SUMO-1氨基末端的氨基酸序列一致的肽段,免疫动物获得。 Western blot analysis of recombinant GST-SUMO-1 protein (38 kDa) and extracts from Jurkat cells using SUMO-1 (C9H1) Rabbit mAb. 使用SUMO-1(C9H1)兔单克隆抗体对重组GST-SUMO-1蛋白(38 kDa)和Jurkat细胞提取物进行免疫印迹分析的结果。 Immunohistochemical analysis of paraffin-embedded human lung carcinoma using SUMO-1 (C9H1) Rabbit mAb in the presence of control peptide (left) or antigen specific peptide (right). 使用SUMO-1(C9H1)兔单克隆抗体对存在对照肽(左)或特定抗原肽(右)的石蜡包埋人肺癌切片进行免疫组化分析的结果。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using SUMO-1 (C9H1) Rabbit mAb in the presence of control peptide (left) or antigen specific peptide (right).

Western Blotting

Western Blotting

Western blot analysis of recombinant GST-SUMO-1 protein (38 kDa) and extracts from Jurkat cells using SUMO-1 (C9H1) Rabbit mAb.

Background

小泛素相关修饰因子1,2和3(SUMO-1,-2和-3)是泛素样蛋白家族(1)的成员。SUMO-1,-2或-3(SUMO化)是类似于泛素化的对目标蛋白的共价修饰。这种翻译后修饰是可逆的、多步骤的过程,在前体蛋白转变为成熟蛋白的裂解过程中启动。成熟的SUMO-1,-2或-3,链接到激活酶E1,再偶联到E2并结合到E3,最终被连接到目标蛋白质(2)。泛素和每个SUMO成员都针对不同的蛋白质具有不同的生物功能。泛素主要调控目标(1)蛋白质降解。相比之下,SUMO-1与RanGAP、PML、P53和IκB-α等蛋白相偶联,调节核转运、亚核结构的形成、转录活性和蛋白质的稳定性(3-7)。 SUMO-2/-3以多聚化偶联的形式,结合拓扑异构酶II和APP,调节染色体分离和环境压力下细胞的反应,并在阿尔茨海默病的发展过程中(8-11)发挥作用。

  1. Schwartz, D.C. and Hochstrasser, M. (2003) Trends Biochem. Sci. 28, 321-8.
  2. Kim, K.I. et al. (2002) J. Cell Physiol. 191, 257-68.
  3. Matunis, M.J. et al. (1996) J. Cell Biol. 135, 1457-70.
  4. Duprez, E. et al. (1999) J. Cell Sci. 112, 381-93.
  5. Gostissa, M. et al. (1999) EMBO J. 18, 6462-74.
  6. Rodriguez, M.S. et al. (1999) EMBO J. 18, 6455-61.
  7. Desterro, J.M. et al. (1998) Mol. Cell 2, 233-9.
  8. Tatham, M.H. et al. (2001) J. Biol. Chem. 276, 35368-74.
  9. Azuma, Y. et al. (2003) J. Cell Biol. 163, 477-87.
  10. Li, Y. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 259-64.
  11. Saitoh, H. and Hinchey, J. (2000) J. Biol. Chem. 275, 6252-8.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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