Cell Signaling Technology

Product Pathways - Autophagy Signaling

Phospho-MFF (Ser146) Antibody #49281

MFF  

No. Size Price
49281S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
49281 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 25, 27 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-MFF (Ser146) Antibody recognizes endogenous levels of MFF protein only when phosphorylated at Ser146. This antibody has been shown to react with phosphorylated Ser146 of human MFF isoforms 2, 4, and 5 but not phosphorylated Ser172 of human MFF isoform 1 (7). This antibody cross-reacts with a 140kDa protein of unknown identity.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser146 of human MFF isoform 2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from MCF7 cells, untreated (-) or treated with CCCP (100μM, 2hr; +), using phospho-MFF (Ser146) Antibody (upper) or total MFF antibody (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from MCF7 cells, untreated (-) or treated with CCCP (100uM, 2hr; +), using Phospho-MFF (Ser146) Antibody. The phospho-specificity of the antibody was verified by treating the membrane with (+) or without (-) calf intestinal phosphatase (CIP) after protein transfer.

Background

Mitochondrial fission factor (MFF) is a tail-anchored protein that resides within the outer mitochondrial membrane and is part of the mitochondrial fission complex. MFF participates in mitochondrial fission by serving as one of multiple receptors for the GTPase dynamin-related protein 1 (Drp1) (1-4). Research studies have also shown that MFF is a peroxisomal membrane protein and participates in peroxisome fission by serving as a receptor for another GTPase, dynamin-like protein 1 (5,6).

Research studies have demonstrated that the ability of MFF to drive acute mitochondrial fission in response to mitochondrial stress is controlled by AMPK-dependent phosphorylation. AMPK directly phosphorylates MFF at two sites to allow for enhanced recruitment of Drp1 to the mitochondra (7). Multiple isoforms of MFF are expressed as a result of alternative splicing (8). One of the major phosphoacceptor sites of MFF (Ser172 of human isoform 1/Ser146 of human isoforms 2-5) lies within an AMPK phsophorylation motif that spans the boundary of differentially spliced exons of MFF isoforms, suggesting that MFF splice isoforms are phosphorylated by AMPK to varying degrees.

  1. Liu, R. and Chan, D.C. (2015) Mol Biol Cell 26, 4466-77.
  2. Shen, Q. et al. (2014) Mol Biol Cell 25, 145-59.
  3. Losón, O.C. et al. (2013) Mol Biol Cell 24, 659-67.
  4. Otera, H. et al. (2010) J Cell Biol 191, 1141-58.
  5. Itoyama, A. et al. (2013) Biol Open 2, 998-1006.
  6. Gandre-Babbe, S. and van der Bliek, A.M. (2008) Mol Biol Cell 19, 2402-12.
  7. Toyama, E.Q. et al. (2016) Science 351, 275-81.
  8. Ducommun, S. et al. (2015) Cell Signal 27, 978-88.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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