Cell Signaling Technology

Product Pathways - Protein Stability

Ubc9 Antibody #4918

p18   SUMO   SUMO-protein ligase   UBCE9   UBE2I   Ubiquitin carrier protein 9   Ubiquitin carrier protein I   Ubiquitin-conjugating enzyme E2 I   Ubiquitin-protein ligase I  

No. Size Price
4918S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
4918 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 17 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Ubc9抗体识别内源性Ubc9总蛋白水平。

Source / Purification

Ubc9多克隆抗体是通过合成与人Ubc9蛋白V148邻近的氨基酸残基序列一致的肽段,免疫动物获得,抗体经肽亲和层析纯化。 Western blot analysis of extracts from various cell lines using Ubc9 Antibody. 使用Ubc9抗体对不同细胞裂解物进行免疫印迹分析的结果。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Ubc9 Antibody.

Background

SUMO-1偶联过程与泛素化修饰和其他翻译后修饰过程相似(1),SUMO-1通过泛素结合酶E1、E2和E3的协同作用而偶联于目标蛋白(2)。Ubc9 (或ube2M)是一个由158氨基酸构成的非常保守的SUMO-1偶联酶(3)。Ubc9通过其SUMO-1-CS (共有序列)结构域结合到靶蛋白上,并通过保守的氨基端结构域与SUMO分子相互作用(3,4)。 定位于核内和核孔的Ubc9可以催化底物蛋白的sumo化修饰并调节靶蛋白的核质转运和转录活性(5,6)。 Ubc9靶蛋白包括RAD51、RAD52、p53和c-Jun,调节细胞周期、DNA损伤和p53介导的其他过程。

  1. Geiss-Friedlander, R. and Melchior, F. (2007) Nat Rev Mol Cell Biol 8, 947-56.
  2. Tatham, M.H. et al. (2003) Biochemistry 42, 3168-79.
  3. Sampson, D.A. et al. (2001) J Biol Chem 276, 21664-9.
  4. Liu, Q. et al. (1999) J Biol Chem 274, 16979-87.
  5. Lee, G.W. et al. (1998) J Biol Chem 273, 6503-7.
  6. Pichler, A. and Melchior, F. (2002) Traffic 3, 381-7.
  7. Mo, Y.Y. and Moschos, S.J. (2005) Expert Opin Ther Targets 9, 1203-16.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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