Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

Phospho-YAP (Ser127) Antibody #4911

65 kDa Yes-associated protein   hippo   P-YAP   YAP65   yaptaz  

No. Size Price
4911S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
4911 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 65 to 75 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Monkey, Bovine,

Specificity / Sensitivity

Phospho-YAP (Ser127) Antibody detects endogenous levels of YAP only when phosphorylated at serine 127.


Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser127 of human YAP. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from MDA-MB-468 cells, untreated or treated with wortmannin and LY294002, using Phospho-YAP (Ser127) Antibody. The phospho-specificity of the antibody was verified by treating the membrane with (+) or without (-) calf intestinal phosphatase (CIP) after western transfer.使用Phospho-YAP (Ser127)对未处理或经过渥曼青霉素和LY294002处理后的MDA-MB-468 细胞提取物进行Western blot分析。本抗体的磷酸化特异性验证通过小肠磷酸酶(CIP)处理或不处理western转膜结束后的膜进行。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, showing cytoplasmic and nuclear localization, using Phospho-YAP (Ser127) Antibody.使用小肠磷酸酶(CIP)处理或不处理对石蜡包埋的人结肠癌组织进行免疫组化,显示细胞质和细胞核定位。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-YAP (Ser127) Antibody in the presence of control peptide (left) or antigen specific peptide (right).在对照多肽(左)和抗原特异性多肽(右)存在的条件下使用Phospho-YAP (Ser127) 对石蜡包埋的人乳腺癌进行免疫组化分析。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma control (left) or lambda phosphatase-treated (right), using Phospho-YAP (Ser127) Antibody使用Phospho-YAP (Ser127) 抗体对对照组(左)或lambda磷酸酶处理后(右)石蜡包埋的人肺癌组织进行免疫组化分析。


YAP (Yes-associated protein, YAP65) was identified based on its ability to associate with the SH3 domain of Yes. It also binds to other SH3 domain-containing proteins such as Nck, Crk, Src, and Abl (1). In addition to the SH3 binding motif, YAP contains a PDZ interaction motif, a coiled-coil domain, and WW domains (2-4). While initial studies of YAP all pointed towards a role in anchoring and targeting to specific subcellular compartments, subsequent studies showed that YAP is a transcriptional co-activator by virtue of its WW domain interacting with the PY motif (PPxY) of the transcription factor PEBP2 and other transcription factors (5,6). Upon phosphorylation at Ser127, YAP interacts with 14-3-3 in an Akt-dependent manner and suppresses p73-mediated apoptosis (6).

YAP蛋白(Yes相关蛋白,YAP65)因其与Yes的SH3结构域结合而被鉴定,它也会结合含有SH3结构域的其他蛋白,如Nck, Crk, Src, 和Abl(1)。除了SH3结合基序,YAP 还含有一个PDZ相互作用基序、卷曲螺旋结构域和WW结构域(2-4)。YAP的早期研究都指出它在锚定和靶定特异细胞组合时起作用,随后还发现YAP是转录共激活因子,其机理主要是是通过YAP的WW结构域与PEBP2转录因子和其他转录因子的PY基序(PPxY)相互作用(5,6)。由于在丝氨酸127的磷酸化,YAP蛋白依赖于AKT的与14-3-3蛋白相互作用,进而抑制p73介导的细胞凋亡(6)。

  1. Sudol, M. (1994) Oncogene 9, 2145-52.
  2. Mohler, P.J. et al. (1999) J Cell Biol 147, 879-90.
  3. Espanel, X. and Sudol, M. (2001) J Biol Chem 276, 14514-23.
  4. Sudol, M. et al. (1995) FEBS Lett 369, 67-71.
  5. Yagi, R. et al. (1999) EMBO J 18, 2551-62.
  6. Basu, S. et al. (2003) Mol Cell 11, 11-23.
  7. Zhao, B. et al. (2010) Genes Dev 24, 862-74.
  8. Zhao, B. et al. (2007) Genes Dev 21, 2747-61.
  9. Yu, F.X. et al. (2012) Cell 150, 780-91.

Application References

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