Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Phospho-Wee1 (Ser642) (D47G5) Rabbit mAb #4910

No. Size Price
4910S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
4910T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价 防伪查询
4910 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 95 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,


Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey, Xenopus, Zebrafish, Bovine,

Specificity / Sensitivity

Phospho-Wee1 (Ser642) (D47G5) Rabbit mAb detects endogenous levels of wee1 protein only when phosphorylated at Ser642.Phospho-Wee1 (Ser642) (D47G5) Rabbit mAb能够检测内源性丝氨酸(642位)磷酸化的wee 1蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser642 of human wee1. 单克隆抗体是由合成的人源的针对wee1蛋白丝氨酸(642位)的磷酸化肽段免疫动物生产的。

Western Blotting

Western Blotting

Western blot analysis of extracts from A431 and H441 cells, untreated or EGF-treated, using Phospho-Wee1 (Ser642) (D47G5) Rabbit mAb (upper) or Wee1 Antibody #4936 (lower).Western blot检测非处理和EGF处理的A431和H441细胞提取物,使用的抗体为Phospho-Wee1 (Ser642) (D47G5) Rabbit mAb (上图) 或Wee1 Antibody #4936 (下图)。



Immunoprecipitation of phospho-wee1 from A431 cells, untreated or EGF-treated, using Phospho-Wee1 (Ser642) (D47G5) Rabbit mAb followed by Western blot using the same antibody.从A431细胞中免疫沉淀磷酸化wee1蛋白,分为非处理组和EGF处理组,使用的抗体为Phospho-Wee1 (Ser642) (D47G5) Rabbit mAb ,随后western blot使用同一抗体。


Entry of all eukaryotic cells into mitosis is regulated by activation of cdc2 kinase. The critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Thr14 (1,2). Phosphorylation at Tyr15 and Thr14 and inhibition of cdc2 is carried out by Wee1 and Myt1 protein kinases, while Tyr15 dephosphorylation and activation of cdc2 is carried out by the cdc25 phosphatase (1,3,4). Hyperphosphorylation and inactivation of Myt1 in mitosis suggests that one or more kinases activated at the G2/M transition negatively regulates Myt1 activity. Kinases shown to phosphorylate Myt1 include cdc2, p90RSK, Akt, and Plk1 (5-8). 所有真核细胞进入有丝分裂都经由cdc2激酶激活的调节。激活cdc2进入有丝分裂进程的关键的调节步骤似乎是15位和14位酪氨酸的去磷酸化(1,2)。15位和14位酪氨酸的磷酸化和cdc2抑制是通过Wee1和MYT1蛋白激酶来执行的,而15位酪氨酸的去磷酸化和cdc2活化是通过cdc25磷酸酶来执行(1,3,4)。有丝分裂中Myt1的过度磷酸化表明G2/ M转换期一个或多个激酶的激活负向调节Myt1活性。已经证实的磷酸化Myt1的激酶包括cdc2,p90RSK,Akt和Plk1 (5-8)。

Akt/PKB-dependent phosphorylation at Ser642 promotes a change in Wee1 localization from nuclear to cytoplasmic and is associated with G2/M arrest (9). Akt/ PKB依赖的642位丝氨酸磷酸化促进了Weel1核质迁移的改变并与G2/ M期阻滞有关(9)。

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  2. Hunter, T. (1995) Cell 80, 225-236.
  3. Galaktionov, K. et al. (1995) Genes Dev 9, 1046-58.
  4. McGowan, C.H. and Russell, P. (1993) EMBO J 12, 75-85.
  5. Booher, R.N. et al. (1997) J Biol Chem 272, 22300-6.
  6. Palmer, A. et al. (1998) EMBO J 17, 5037-47.
  7. Nakajima, H. et al. (2003) J Biol Chem 278, 25277-80.
  8. Katayama, K. et al. (2005) Mol Cell Biol 25, 5725-37.

Application References

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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