Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Phospho-cdc25C (Ser216) (63F9) Rabbit mAb #4901

No. Size Price
4901S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
4901T 20 µl ( 2 western blots ) ¥1,300.00 现货查询 购买询价
4901 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey, Endogenous 60 Rabbit IgG
IP 1:50
IHC-P 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

Phospho-cdc25C (Ser216) (63F9) Rabbit mAb detects endogenous levels of cdc25C only when phosphorylated at Ser216. Phospho-cdc25C (Ser216) (63F9) Rabbit mAb 能够检测内源性丝氨酸(216位点)磷酸化的cdc25C蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser216 of human cdc25C. 该单克隆抗体是有合成的人源的针对丝氨酸(216位点)的磷酸化肽段免疫动物生产的。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, showing cytoplasmic localization, using Phospho-cdc25C (Ser216) (63F9) Rabbit mAb. 免疫组织化学方法检测石蜡包埋人类结肠癌组织,显示胞浆定位,使用的抗体为Phospho-cdc25C (Ser216) (63F9) Rabbit mAb。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-cdc25C (Ser216) (63F9) Rabbit mAb. 免疫组织化学分析石蜡包埋人类乳腺癌组织,使用的抗体为Phospho-cdc25C (Ser216) (63F9) Rabbit mAb。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-cdc25C (Ser216) (63F9) Rabbit mAb. 免疫组织化学分析石蜡包埋人类肺癌组织,使用的抗体为Phospho-cdc25C (Ser216) (63F9) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, untreated (left) or lambda phosphatase-treated (right), using Phospho-cdc25C (Ser216) (63F9) Rabbit mAb. 免疫组织化学分析石蜡包埋人类肺癌组织,左图为未处理组织,右图为lambda磷酸酶处理的组织,使用的抗体为Phospho-cdc25C (Ser216) (63F9) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lymph node, using Phospho-cdc25C (Ser216) (63F9) Rabbit mAb in the presence of control peptide (left) or Phospho-cdc25C (Ser216) Blocking Peptide #1190 (right). 免疫组织化学分析石蜡包埋人类淋巴结组织,左图为对照多肽,右图为Phospho-cdc25C (Ser216) Blocking Peptide 多肽#1190 ,使用的抗体为Phospho-cdc25C (Ser216) (63F9) Rabbit mAb 。

Western Blotting

Western Blotting

Western blot analysis of GST-cdc25C, unphosphorylated or phosphorylated in vitro by Chk2, using Phospho-cdc25C (Ser216) (63F9) Rabbit mAb (upper) or cdc25C antibody #9522 (lower).

Western Blotting

Western Blotting

Western Blot analysis of HT29 cells, untreated, nocodazole-treated, and lambda phosphotase-treated, using Phospho-cdc25C (Ser216) antibody (upper), and cdc25C (5H9) Rabbit Mab, #4688, (lower). western blot方法检测未处理、nocodazole处理和λ磷酸酶处理的HT29细胞提取物,使用的抗体为Phospho-cdc25C (Ser216) antibody (上图)和cdc25C (5H9) Rabbit Mab, #4688, (下图)。

Background

cdc25 is a protein phosphatase responsible for dephosphorylating and activating cdc2, a crucial step in regulating the entry of all eukaryotic cells into mitosis (1). cdc25C is constitutively phosphorylated at Ser216 throughout interphase by c-TAK1, while phosphorylation at this site is DNA damage-dependent at the G2/M checkpoint (2). When phosphorylated at Ser216, cdc25C binds to members of the 14-3-3 family of proteins, sequestering cdc25C in the cytoplasm preventing premature mitosis (3). The checkpoint kinases Chk1 and Chk2 phosphorylate cdc25C at Ser216 in response to DNA damage (4,5). cdc25蛋白磷酸酶负责cdc2的去磷酸化和激活,该过程是真核细胞进入有丝分裂的关键调节步骤(1)。整个分裂间期cdc25C丝氨酸216位发生c-TAK1引起的组成性磷酸化,而该位点的磷酸化在G2/M期检验点是不依赖DNA损伤的(2)。当216位丝氨酸磷酸化时,cdc25C结合14-3-3蛋白家族的成员,扣押胞质cdc25C从而阻止有丝分裂的过早发生(3)。检验点蛋白激酶Chk1和Chk2磷酸化cdc25C216位丝氨酸以响应DNA损伤(4,5)。

  1. Jessus, C. and Ozon, R. (1995) Prog. Cell Cycle Res. 1, 215-228.
  2. Peng, C.Y. et al. (1997) Science 277, 1501-1505.
  3. Kumagai, A. and Dunphy, W.G. (1999) Genes Dev. 13, 1067-1072.
  4. Blasina, A. et al. (1999) Curr. Biol. 9, 1-10.
  5. Furnari, B. et al. (1999) Mol. Biol. Cell 10, 833-845.

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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