Cell Signaling Technology

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Ajuba Antibody #4897

ajuba homolog   isoform 1   Jub   JUB protein   jub. ajuba homolog {JUB}  

No. Size Price
4897S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
4897 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Monkey, Endogenous 55 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,


Species predicted to react based on 100% sequence homology: Rat,

Specificity / Sensitivity

Ajuba Antibody detects endogenous levels of total Ajuba protein.


Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human Ajuba. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from P19 cells (lane 1) and COS-7 cells transfected with Ajuba (lane 2), using Ajuba Antibody.对P19细胞(第一道)和转染了Ajuba的COS-7细胞(第二道)提取物使用Ajuba抗体进行western blot分析。

Western Blotting

Western Blotting

Western blot analysis of extracts from MEF -/- cells rescued with a construct overexpressing Ajuba (lane 1), MEF -/- cells (lane 2), MEF +/+ cells (lane 3) and P19 cells (lane 4), using Ajuba Antibody. (Kindly provided by Dr. Gregory D. Longmore, Washington University, St. Louis, Missouri.)过表达Ajuba进行挽救实验的MEF-/-细胞(第1道),MEF -/- 细胞 (第2道), MEF +/+ 细胞cells (第 3道)和P19细胞(第4道)提取物使用Ajuba抗体进行western blot分析(由密苏里州圣路易斯华盛顿大学Gregory D. Longmore博士友情提供)。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of COS cells, using Ajuba antibody (blue) compared to a non-specifc negative control antibody (red).使用Ajuba抗体(蓝色)与非特异性阴性对照抗体(红色)对COS细胞进行流式分析。


Ajuba belongs to the Zyxin/Ajuba family, members of which include LIMD1, LPP, Trip6 and Zyxin. Three LIM domains at the carboxy-terminus characterize this family of proteins. Zyxin/Ajuba family members associate with the actin cytoskeleton, are components of both cell-cell junction adhesive complex and integrin-mediated adhesive complex, and shuttle in and out of the nucleus (1). Ajuba is involved in Rac activation during cell migration and activates Aurora A in order to commit to mitosis (2-3). Ajuba and its family members also have an amino-terminal proline-rich pre-LIM region, and Ajuba augments ERK activation in a Ras-dependent manner (4).

Ajuba属于Zyxi/Ajuba家族,该家族成员包括LIMD1,LPP,Trip6和Zyxin。该家族蛋白具有共同的特点在羧基端带有3个LIM结构域。Zyxin/Ajuba家族成员与细胞骨架肌动蛋白丝结合,成为细胞与细胞间连接复合物和整联蛋白介导的粘附复合物的组成成分,并在细胞核穿梭(1)。Ajuba涉及到细胞迁移时Rac的激活,并且激活Aurora A以便使细胞进入有丝分裂状态(2-3)。Ajuba和它的家族成员也在氨基端拥有富含脯氨酸的pre-LIM区域,Ajuba会在Ras-依赖的状态下促进ERK激活(4)。

  1. Feng, Y. and Longmore, G.D. (2005) Mol. Cell Biol. 25, 4010-4022.
  2. Pratt, S. J. et al. (2005) J. Cell Biol. 168, 813-824.
  3. Hirota, T. et al. (2003) Cell 114, 585-598.
  4. Goyal, R. et al. (1999) Mol. Cell Biol. 19, 4379-4389.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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