Cell Signaling Technology

Product Pathways - Protein Stability

UBE1a/b Antibody #4891

A1S9 protein   E1enzyme   UBE   Ubiquitin activating enzyme E1  

No. Size Price
4891S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
4891 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 110, 117 Rabbit
IF-IC 1:25

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

UBE1a/b抗体识别内源性的UBE1a和UBE1b蛋白。

Source / Purification

UBE1a/b多克隆抗体是通过合成与人UBE1蛋白Thr800邻近的氨基酸序列一致的肽段,免疫动物获得,抗体经Protein A和肽亲和层析纯化。 Western blot analysis of extracts from K562 and SK-N-MC cells using UBE1a/b Antibody. 使用UBE1a/b抗体对K562和SK-N-MC细胞裂解物进行免疫印迹分析的结果。 Confocal immunofluorescent analysis of SK-N-MC cells using UBE1a/b Antibody (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). 使用UBE1a/ B抗体检测SK-N-MC细胞的共聚焦免疫荧光分析结果(绿色),蓝色伪彩为 DRAQ5™(DNA荧光染料)。

IF-IC

IF-IC

Confocal immunofluorescent analysis of SK-N-MC cells using UBE1a/b Antibody (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of extracts from K562 and SK-N-MC cells using UBE1a/b Antibody.

Background

泛素是一个在泛素——蛋白酶体途径中发挥重要作用的保守多肽。泛素能够通过泛素转移过程被共价修饰于多个细胞蛋白,导致蛋白通过26S蛋白酶体降解。三个泛素化酶参与泛素偶联过程:泛素首先与活化酶E1(UBE1或E1)形成硫酯键复合体而被激活,然后被转移到泛素载体蛋白E2,然后由E2转移到E3并连接到靶蛋白赖氨酸的epsilon位氨基上(1-3),不同E2和E3的组合决定底物特异性(4)。UBE1有两个亚型:UBE1a是一个117kDa的核蛋白,UBE1b是一个110kDa的核质蛋白(5)。

  1. Ciechanover, A. (1998) EMBO J. 17, 7151-7160.
  2. Hochstrasser, M. (2000) Nat. Cell Biol. 2, E153-E157.
  3. Hochstrasser, M. (2000) Science 289, 563-564.
  4. DeSalle, L.M. and Pagano, M. (2001) FEBS Lett. 490, 179-189.
  5. Stephen, A.G. et al. (1996) J. Biol. Chem. 271, 15608-15614.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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