Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

KLC1 (D2T2R) Rabbit mAb #48633

sc-13361   sc-25735  

No. Size Price
48633S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
48633 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 65, 75 Rabbit IgG
IP 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

KLC1 (D2T2R) Rabbit mAb recognizes endogenous levels of total KLC1 protein. This antibody also cross-reacts with an unidentified protein of 25 kDa.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly443 of human KLC1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using KLC1 (D2T2R) Rabbit mAb.



Immunoprecipitation of KLC1 from HT-29 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is KLC1 (D2T2R) Rabbit mAb. Western blot was performed using KLC1 (D2T2R) Rabbit mAb.


Kinesins are heterotetrameric motor proteins that transport cargo along microtubule tracks toward their plus ends (anterograde direction) in an ATP-dependent manner. Two heavy chains contain the motor activity, while two kinesin light chains act as adaptor proteins that may be required for binding of specific cargo and/or regulation of heavy chain catalytic activity. The amino terminus of kinesin light chain 1 (KLC1) binds to kinesin heavy chains while the KLC1 carboxy-terminal tetratricopeptide repeat (TPR) domain binds cargo (1-3). Phosphorylation of KLC1 at Ser521 by AMPK may regulate insulin granule dynamics (4,5). Research studies identify a KLC1-ALK fusion protein in human lung adenocarcinoma, and this finding may provide insight for future therapeutics (6).

  1. Verhey, K.J. and Hammond, J.W. (2009) Nat Rev Mol Cell Biol 10, 765-77.
  2. Hirokawa, N. et al. (2009) Nat Rev Mol Cell Biol 10, 682-96.
  3. Kawano, T. et al. (2012) Traffic 13, 834-48.
  4. McDonald, A. et al. (2010) Biochem Soc Trans 38, 205-8.
  5. McDonald, A. et al. (2009) Islets 1, 198-209.
  6. Togashi, Y. et al. (2012) PLoS One 7, e31323.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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