Cell Signaling Technology

Product Pathways - Adhesion

Phospho-Zyxin (Ser142/143) Antibody #4863

ESP-2   HED-2   p83  

No. Size Price
4863S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
4863 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Monkey, Endogenous 78 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-Zyxin (Ser142/143) Antibody recognizes endogenous levels of zyxin protein only when phosphorylated at Ser142, Ser143 or both residues.

Phospho-Zyxin (Ser142/143)抗体仅能识别142位,143位丝氨酸磷酸化或两个氨基酸残基同时同时磷酸化的内源性zyxin蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser142 of human zyxin protein. Antibodies are purified using protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from A-431 cells, untreated (-) or EGF-treated (30 min; +), using Phospho-Zyxin (Ser142/143) Antibody (upper) and Zyxin Antibody #3553 (lower).未处理(-)或经过EGF处理(30分钟;+)的A-431细胞提取物,使用Phospho-Zyxin (Ser142/143)抗体(上)和Zyxin抗体#3553(下)进行western blot分析。


The zyxin family of proteins includes LIMD1, ajuba, trip6, and zyxin, each of which contains three LIM domains at the carboxy terminus. Zyxin family members associate with the actin cytoskeleton and are components of both the cell-cell junction adhesive complex and the integrin-mediated adhesive complex. They shuttle in and out of the nucleus where they may function in transcriptional activation (1). Zyxin is involved in the regulation of mechanical force-induced actin polymerization at focal adhesions (2) and in the regulation of adhesion and migration, possibly through the recruitment of Ena/VASP proteins to focal adhesions (3). Zyxin interacts with and may regulate the function of the tumor suppressor myopodin, which inhibits tumor growth and metastasis (4). Zyxin may be phosphorylated by Akt at Ser142 (5).

zyxin (斑联蛋白)家族包括LIMD1,ajuba,trip6和zyxin ,每个蛋白在羧基末端都有3个LIM结构域。Zyxin 家族成员与肌动蛋白丝细胞骨架相关联,是细胞间连接和整联蛋白介导的粘附复合物的组成部分。它们在细胞核内外穿梭发挥转录激活作用(1)。Zyxin涉及调控肌动蛋白丝机械压力诱导下在黏着斑部位的多聚化(2)并且调控黏附和迁徙,可能是通过向黏着斑募集Ena/VASP蛋白而实现的(3)。Zyxin与肿瘤抑制因子myopodin结合并可能调控器功能,myopodin能够抑制肿瘤生长和转移(4)。Zyxin可以被Akt在第142位丝氨酸磷酸化(5)。

  1. Wang, Y. and Gilmore, T.D. (2003) Biochim Biophys Acta 1593, 115-20.
  2. Hirata, H. et al. (2008) J Cell Sci 121, 2795-804.
  3. Hoffman, L.M. et al. (2006) J Cell Biol 172, 771-82.
  4. Yu, Y.P. and Luo, J.H. (2006) Cancer Res 66, 7414-9.

Application References

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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