Cell Signaling Technology

Product Pathways - Protein Translation

Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb #4857

ps6  

No. Size Price
4857S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
4857 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 32 Rabbit IgG
IHC-P 1:75
IF-IC 1:100
IHC-F 1:75

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), IHC-F=Immunohistochemistry (Frozen),

Specificity / Sensitivity

Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb detects endogenous levels of ribosomal protein S6 only when phosphorylated at serines 235 and 236.

Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb兔单抗检测仅在丝氨酸235和236位点磷酸化的内源性核糖体蛋白S6水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser235 and Ser236 of human ribosomal protein S6.

通过人工合成人源核糖体蛋白S6的Ser235和Ser236位点周围相应的磷酸化片段去免疫动物从而制备单克隆抗体。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, showing cytoplasmic localization, using Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb.

使用Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) 兔单抗,免疫组化分析人类结肠癌石蜡切片,结果显示磷酸化S6核糖体蛋白 (Ser235/236)蛋白在细胞质定位。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb in the presence of control peptide (left) or Phospho-S6 Ribosomal Protein (Ser235/236) Blocking Peptide #1220 (right).

使用Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb兔单抗,免疫组化分析人类肺癌石蜡切片,其分别孵育对照多肽(左图)和Phospho-S6 Ribosomal Protein (Ser235/236) Blocking Peptide #1220 (右图)。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded LNCaP cells, untreated (left) or rapamycin-treated (right), using Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb.

使用Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb兔单抗,免疫组化分析LNCaP细胞石蜡切片,切片分为对照组(左图)和rapamycin处理组(右图)。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb.

使用Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb兔单抗,免疫组化分析人类乳腺癌组织石蜡切片。

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, untreated or PDGF-treated (100ng/ml, 20 min), using Phospho-S6 Ribosomal protein (Ser235/236) (91B2) Rabbit mAb (upper) or S6 Ribosomal Protein (5G10) Rabbit mAb #2217 (lower).

使用Phospho-S6 Ribosomal protein (Ser235/236) (91B2) Rabbit mAb兔单抗 (上图)和S6 Ribosomal Protein (5G10) Rabbit mAb兔单抗 #2217 (下图),免疫印迹(Western Blot)分析在NIH/3T3细胞系中Phospho-S6 Ribosomal Protein (Ser235/236)和S6 Ribosomal Protein兔单抗 (5G10)蛋白水平,分为对照组和PDGF处理组(100 ng/ml, 20分钟)。

IHC-F (frozen)

IHC-F (frozen)

Immunohistochemical analysis of frozen U-87MG xenograft, showing cytoplasmic localization using Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb.

使用Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb兔单抗,免疫组化分析U-87MG异种移植物的冰冻切片,结果显示磷酸化S6核糖体蛋白 (Ser235/236)在细胞质定位。

IF-IC

IF-IC

Confocal immunofluorescent images of HeLa cells serum-starved for 20 hrs (left), 20% serum-treated (center), or 20% serum-treated after preincubation with Rapamycin (FRAP/mTOR Inhibitor) #9904 and labeled with Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb (red). Actin filaments have been labeled with fluorescein phalloidin. Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

Background

One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions (2). These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5).

生长因子和有丝分裂原有效促进维持细胞生长和增殖的一个方面就是通过上调mRNA翻译(1,2)。生长因子和有丝分裂原能诱导p70 S6激酶的激活和随后S6核糖体蛋白的磷酸化。S6核糖体蛋白的磷酸化与mRNA转录本的翻译增加有关,而这个mRNA转录本是在5端非翻译区含有一个寡嘧啶区(2)。这些特殊的mRNA转录本 (5'TOP) 能够编码涉及细胞周期进程的蛋白质如翻译必须的核糖体蛋白和延伸因子(2,3)。重要的S6核糖体蛋白的磷酸化位点包含数个定位在S6蛋白一个小的羧基末端区域残基(Ser235, Ser236, Ser240, and Ser244) (4,5)。

  1. Dufner, A. and Thomas, G. (1999) Exp Cell Res 253, 100-9.
  2. Peterson, R.T. and Schreiber, S.L. (1998) Curr Biol 8, R248-50.
  3. Jefferies, H.B. et al. (1997) EMBO J 16, 3693-704.
  4. Ferrari, S. et al. (1991) J Biol Chem 266, 22770-5.
  5. Flotow, H. and Thomas, G. (1992) J Biol Chem 267, 3074-8.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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