Cell Signaling Technology

Product Pathways - Protein Translation

Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb #4856


No. Size Price
4856S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
4856 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 32 Rabbit IgG
F 1:200
IF-IC 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb detects endogenous levels of ribosomal protein S6 only when phosphorylated at serine 235 and 236.

Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb兔单抗检测仅在丝氨酸235和236位点磷酸化的内源性核糖体蛋白S6。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser235 and Ser236 of human ribosomal protein S6.


Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, untreated or PDGF-treated (100 ng/ml, 20 min), using Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (upper) or S6 Ribosomal Protein (5G10) Rabbit mAb #2217 (lower).

使用Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb兔单抗 (上图)和S6 Ribosomal Protein (5G10) Rabbit mAb兔单抗 #2217 (下图),免疫印迹(Western Blot)分析在NIH/3T3细胞系中磷酸化S6 核糖体蛋白(Ser235/236)和S6核糖体蛋白(5G10)蛋白水平,实验分为对照组和PDGF处理组(100 ng/ml, 20分钟)。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, untreated (green), or LY294002, Wortmannin and U0126-treated (blue), using Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb compared to a nonspecific negative control antibody (red).

与阴性非特异的对照抗体(红色)比较,使用Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb兔单抗,流式细胞术分析Jurkat细胞,细胞分为未处理组(绿色)和LY294002处理组(蓝色)。



Confocal immunofluorescent images of HeLa cells serum-starved for 20 hrs (left), 20% serum-treated (center), or 20% serum-treated after preincubation with Rapamycin (FRAP/mTOR Inhibitor) #9904 and labeled with Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

使用Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb兔单抗(绿色)标记,共聚焦免疫荧光观察磷酸化S6 核糖体蛋白在HeLa细胞中的定位,细胞分别经血清饥饿培养20小时(左图),20%血清处理(中图)或在Rapamycin (FRAP/mTOR Inhibitor) #9904孵育后加20%血清处理(右图)。DY-554 phalloidin标记微丝蛋白(红色)。蓝色伪彩 = DRAQ5™ (DNA 荧光染料).


One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions (2). These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5).

生长因子和有丝分裂原有效促进维持细胞生长和增殖的一个方面就是通过上调mRNA翻译(1,2)。生长因子和有丝分裂原能诱导p70 S6激酶的激活和随后S6核糖体蛋白的磷酸化。S6核糖体蛋白的磷酸化与mRNA转录本的翻译增加有关,而这个mRNA转录本是在5端非翻译区含有一个寡嘧啶区(2)。这些特殊的mRNA转录本 (5'TOP) 能够编码涉及细胞周期进程的蛋白质如翻译必须的核糖体蛋白和延伸因子(2,3)。重要的S6核糖体蛋白的磷酸化位点包含数个定位在S6蛋白一个小的羧基末端区域残基(Ser235, Ser236, Ser240, and Ser244) (4,5)。

  1. Dufner, A. and Thomas, G. (1999) Exp Cell Res 253, 100-9.
  2. Peterson, R.T. and Schreiber, S.L. (1998) Curr Biol 8, R248-50.
  3. Jefferies, H.B. et al. (1997) EMBO J 16, 3693-704.
  4. Ferrari, S. et al. (1991) J Biol Chem 266, 22770-5.
  5. Flotow, H. and Thomas, G. (1992) J Biol Chem 267, 3074-8.
  6. Kotecha, N. et al. (2008) Cancer Cell 14, 335-43.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

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