Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Phospho-MARK Family (Activation Loop) Antibody #4836

No. Size Price
4836S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
4836 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 80 to 95 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-MARK Family (Activation Loop) Antibody detects endogenous levels of phosphorylated MARK family members, MARK1 at threonine 215, MARK2 at threonine 208, and MARK3 at threonine 234 (A.K.A. 211 in isoforms 3-6) . This antibody does not react with MARK4.

Phospho-MARK Family (Activation Loop) 抗体识别内源性的磷酸化MARK家族成员,MARK1蛋白threonine 215位点、MARK2蛋白threonine 208位点和MARK3蛋白threonine 234位点(A.K.A. 211 in isoforms 3-6)。该抗体不与MARK4反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding threonine 215 of human MARK1. Antibodies were purified by protein A and peptide affinity chromatography.

通过合成与人源MARK1蛋白threonine 215邻近氨基酸序列一致的肽段,免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells transfected with either wild-type or threonine to alanine mutations at the respective phosphorylation sites of the MARK family members, using Phospho-MARK Family (Activation Loop) Antibody. Transfected constructs contain a GST and HA tag. Transfection efficiency monitored with HA Antibody, #2367.

使用Phospho-MARK Family (Activation Loop) Antibody,免疫印迹(Western Blot)分析293细胞Phospho-MARK蛋白水平,细胞分别转染wild-type或转染MARK蛋白家族磷酸化位点苏氨酸突变成丙氨酸。转染的质粒含有一个GST和HA标签。使用HA Antibody, #2367检测转染效率。

Western Blotting

Western Blotting

Western blot analysis of extracts from Raji and BaF3 cells, showing endogenous levels of phosphorylated MARK family members, using Phospho-MARK Family (Activation Loop) Antibody.

使用Phospho-MARK Family (Activation Loop) Antibody,免疫印迹(Western Blot)分析Raji and BaF3细胞Phospho-MARK蛋白水平,结果显示内源性磷酸化MARK蛋白家族。

Background

Microtubule associated proteins regulate the stability of microtubules and control processes such as cell polarity/differentiation, neurite outgrowth, cell division and organelle trafficking (1). The MARK (MAP/microtubule affinity-regulating kinases) family (MARK1-4) of serine/threonine kinases was identified based on their ability to phosphorylate microtubule-associated proteins (MAPs) including tau, MAP2 and MAP4 (2-6). MARK proteins phosphorylate MAPs within their microtubule binding domains, causing dissociation of MAPs from microtubules and increased microtubule dynamics (2-4). In the case of tau, phosphorylation has been hypothesized to contribute to the formation of neurofibrillary tangles observed in Alzheimer's disease. Overexpression of MARK leads to hyperphosphorylation of MAPs, morphological changes and cell death (4). The tumor suppressor kinase LKB1 phosphorylates MARK and the closely related AMP-kinases within their T-loops, leading to increased activity (7).

微管相关蛋白调节微管的稳定性和控制相关进程例如细胞极化/分化、轴突生长、细胞分裂和细胞器的运输(1)。丝氨酸/苏氨酸激酶的MARK (MAP/microtubule affinity-regulating kinases)家族(MARK1-4)被鉴定基于它们的有能力去磷酸化microtubule-associated proteins (MAPs)包括tau、MAP2和MAP4蛋白(2-6)。MARK蛋白在它们的微管结合区域里磷酸化MAPs,这就引起MAPs蛋白从微管上分离,并且增加了微管的动力(2-4)。至于tau蛋白,在阿尔茨海默病人中观察其磷酸化已经被假设有助于神经纤维缠结的形成。MARK激酶的过表达导致MAPs的过磷酸化 、形态学改变和细胞死亡(4)。肿瘤抑制激酶LKB1磷酸化MARK激酶和紧密相关的含有T-loops的AMP-kinases ,导致活性的增加(7)。

  1. Drubin, D.G. and Nelson, W.J. (1996) Cell 84, 335-44.
  2. Illenberger, S. et al. (1996) J Biol Chem 271, 10834-43.
  3. Drewes, G. et al. (1995) J Biol Chem 270, 7679-88.
  4. Drewes, G. et al. (1997) Cell 89, 297-308.
  5. Kato, T. et al. (2001) Neoplasia 3, 4-9.
  6. Trinczek, B. et al. (2004) J Biol Chem 279, 5915-23.
  7. Lizcano, J.M. et al. (2004) EMBO J 23, 833-43.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications