Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

MacroH2A1.2 Antibody #4827

H2A   H2a.y   h2a1   H2AFY   histone variant   macroh2A   macroh2a1  

No. Size Price
4827S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
4827 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 40 Rabbit
IF-IC 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry),

Homology

Species predicted to react based on 100% sequence homology: Chicken, Bovine,

Specificity / Sensitivity

MacroH2A1.2 Antibody detects endogenous levels of the core histone MacroH2A1.2 protein (MacroH2A1, isoform 2). The antibody does not cross-react with MacroH2A1.1 (MacroH2A1, isoform 1), MacroH2A2 or histone H2A.

MacroH2A1.2 Antibody能够检测内源性的histone MacroH2A1.2 (macroH2A1, isoform 2)蛋白。该抗体不能与macroH2A1.2 (macroH2A1, isoform 1), macroH2A2或histone H2A发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the human MacroH2A1.2 protein (MacroH2A1, isoform 2). Antibodies are purified by protein A and peptide affinity chromatography.

通过合成的与人源macroH2A1.2蛋白(macroH2A1, isoform 2)去免疫动物从而制备出此多克隆抗体。通过蛋白A和多肽亲和层析纯化获得。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, H-4-II-E and COS cells using MacroH2A1.2 Antibody.

使用MacroH2A1.2 Antibody,免疫印迹(Western blot)分析HeLa、H-4-II-E和COS细胞系中MacroH2A1.2的蛋白水平。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, either untransfected or transfected with expression constructs for MacroH2A1.1 or MacroH2A1.2, using MacroH2A1.2 Antibody (upper) and MacroH2A1.1 Antibody #4160 (lower).

使用MacroH2A1.2 Antibody (上图)和MacroH2A1.1 Antibody #4160(下图),免疫印迹(Western blot)显示了在HeLa细胞中转染MacroH2A1.2的蛋白水平,细胞分为未转染(-)和转染MacroH2A1.1 或者 MacroH2A1.2 (+)质粒。

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using MacroH2A1.2 Antibody (green). Actin filaments were labeled using DY-554 phalloidin (red).

使用MacroH2A1.2 Antibody(绿色),共聚焦免疫荧光分析HeLa细胞。DY-554 phalloidin标记微丝蛋白(红色)。

Background

Histone macroH2A1 and macroH2A2 comprise a family of variant histone H2A proteins. MacroH2A1 exists as two distinct isoforms due to alternative splicing of a single gene; macroH2A1.1 levels accumulate throughout differentiation and development while macroH2A1.2 shows a constant level of expression (1). MacroH2A1 and macroH2A2 are encoded by completely distinct genes located on separate chromosomes (2,3). Both macroH2A1 and macroH2A2 proteins contain an amino-terminal histone-like region with 64% sequence identity to canonical histone H2A, in addition to a carboxy-terminal “macro” domain (1-3). MacroH2A1 and macroH2A2 are enriched in facultative heterochromatin, including inactivated X chromosomes in mammalian females and senescence-associated heterochromatin foci (2-5). Both act to repress gene transcription by inhibiting the binding of transcription factors to chromatin, the acetylation of histones by p300, and the chromatin-remodeling activities of SWI/SNF and ACF (6,7). The macro domain of macroH2A1.1 binds to ADP-ribose and functions to recruit macroH2A1.1 to activated PARP at sites of DNA damage, where it mediates chromatin rearrangements to locally regulate the DNA damage response (8). MacroH2A1.2 and macroH2A2 do not bind poly-ADP-ribose and are not recruited to sites of activated PARP (8).

Histone macroH2A1和macroH2A2包含多样histone H2A蛋白家族。MacroH2A1存在两种明显的亚型,这是因为一个单独基因的选择性剪切;macroH2A1.1水平在分化和发育阶段中堆积,而macroH2A1.2显示一个恒定的表达水平(1)。MacroH2A1和macroH2A2是通过定位在不同的染色体上的完全不同的基因编码的(2,3)。除了一个羧基端“macro”区域,macroH2A1和macroH2A2蛋白都包含一个氨基端组蛋白样区域,其含有64%序列相似与histone H2A(1-3)。MacroH2A1和macroH2A2在兼性异染色质( facultative heterochromatin)是丰富的,包括在雌性哺乳动物中的失活的X染色体和细胞老化相关异染色质位点(senescence-associated heterochromatic foci, SAHF)(2-5)。它们都可以通过抑制转录因子结合到染色质、由p300使组蛋白乙酰化以及SWI/SNF和ACF的染色质重构活性从而抑制基因转录(6,7)。macroH2A1.1的macro区域结合到ADP-ribose,以及作为在DNA损伤的位点招募macroH2A1.1去激活PARP,在上述区域它能介导染色质重排去特异性调节DNA损伤反应(8)。MacroH2A1.2和macroH2A2不能结合到poly-ADP-ribose,并且不能招募到活性PARP的位点(8)。

  1. Pehrson, J.R. et al. (1997) J Cell Biochem 65, 107-13.
  2. Chadwick, B.P. and Willard, H.F. (2001) Hum Mol Genet 10, 1101-13.
  3. Costanzi, C. and Pehrson, J.R. (2001) J Biol Chem 276, 21776-84.
  4. Costanzi, C. and Pehrson, J.R. (1998) Nature 393, 599-601.
  5. Zhang, R. et al. (2005) Dev Cell 8, 19-30.
  6. Angelov, D. et al. (2003) Mol Cell 11, 1033-41.
  7. Doyen, C.M. et al. (2006) Mol Cell Biol 26, 1156-64.
  8. Timinszky, G. et al. (2009) Nat Struct Mol Biol 16, 923-9.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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