Cell Signaling Technology

Product Pathways - Neuroscience

CREB (D76D11) Rabbit mAb #4820

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No. Size Price
4820S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
4820 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Hamster,Monkey,D. melanogaster, Endogenous 43 Rabbit IgG
IP 1:50
F 1:200
IF-F 1:600
IF-IC 1:600
ChIP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry, IF-F=Immunofluorescence (Frozen), IF-IC=Immunofluorescence (Immunocytochemistry), ChIP=Chromatin IP,

Specificity / Sensitivity

CREB (D76D11) Rabbit mAb detects endogenous levels of total CREB protein. This antibody cross-reacts with ATF-1.

CREB (D76D11)Rabbit mAb 兔单抗识别内源性的总CREB蛋白。该抗体与ATF-1有交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a full length GST-CREB fusion protein.

该单克隆抗体用GST-CREB全长融合蛋白免疫动物而制成。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using CREB (D76D11) Rabbit mAb.

Western blot分析多个细胞系提取物,使用的抗体是CREB (D76D11) Rabbit mAb 兔单抗。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 293 cells treated with Forskolin #3828 (30 µM) and either 10 μl of CREB (D76D11) XP® Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human ALS2 exon 1 primers, SimpleChIP® Human Nr4A3 Promoter Primers #4829, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

使用SimpleChIP®Enzymatic Chromatin IP Kit (Magnetic Beads) #9003试剂盒,将Forskolin #3828 (30 µM)处理1小时后的4 x 10^6 293细胞的染色质,与10 μl CREB (D76D11) XP® Rabbit mAb 兔单抗或者2 μl of Normal Rabbit IgG #2729进行染色质免疫沉淀。使用人ALS2 外显子1的primers、SimpleChIP® Human NR4A3 Promoter Primers #4829和SimpleChIP® Human α Satellite Repeat Primers #4486进行real-time PCR用来定量富集的DNA。每个样本中被沉淀的DNA量用总投入量(等于1)的百分比表示。

IF-IC

IF-IC

Confocal immunofluorescent analysis of SH-SY5Y cells using CREB (D76D11) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).

共聚焦免疫荧光分析SH-SY5Y细胞,使用CREB (D76D11) Rabbit mAb 兔单抗(绿色)进行标记。肌动蛋白纤丝用DY-554 phalloidin(红色)标记。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of SH-SY5Y cells, using CREB (D76D11) Rabbit mAb Antibody (blue) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red).

流式细胞仪分析SH-SY5Y细胞,使用的抗体是CREB (D76D11) Rabbit mAb 兔单抗(蓝色)和对应的Rabbit (DA1E) mAb IgG XP® Isotype Control #3900(红色)。

IF-F

IF-F

Confocal immunofluorescent analysis of mouse brain using CREB (D76D11) Rabbit mAb (green) and β3-Tubulin (TU-20) Mouse mAb #4466 (red).

共聚焦免疫荧光分析小鼠大脑,使用CREB (D76D11) Rabbit mAb 兔单抗(绿色)和β3-Tubulin (TU-20) Mouse mAb 鼠单抗#4466(红色)进行标记。

Background

CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth, and neuronal differentiation in certain neuronal populations (1-3). Additionally, CREB signaling is involved in learning and memory in several organisms (4-6). CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+, and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV, and MAPKAPK-2 (7-9).

CREB的是一个bZIP转录因子,通过cAMP反应元件激活靶基因。CREB能够介导众多的生理刺激信号,调节很多的细胞反应。CREB的在许多组织中表达,它神经系统起着一个重要的调节作用。CREB被认为在促进特定神经元的存活、增殖、突起生长和神经细胞分化中发挥了关键作用(1-3)。此外,CREB信号在数种生物体中涉及学习和记忆(4-6)。CREB能够通过与不同二聚体相互作用,选择性地激活众多的下游基因。CREB因Ser133的磷酸化而激活,激活可由多种信号通路完成,包括ERK,Ca2+和压力信号。CREB在Ser133的磷酸化所涉及激酶包括p90RSK,MSK,CaMKIV, MAPKAPK-2(7-9)。

  1. Lonze, B.E. et al. (2002) Neuron 34, 371-85.
  2. Lee, M.M. et al. (1999) J Neurosci Res 55, 702-12.
  3. Redmond, L. et al. (2002) Neuron 34, 999-1010.
  4. Dash, P.K. et al. (1990) Nature 345, 718-21.
  5. Yin, J.C. et al. (1994) Cell 79, 49-58.
  6. Guzowski, J.F. and McGaugh, J.L. (1997) Proc Natl Acad Sci USA 94, 2693-8.
  7. Xing, J. et al. (1998) Mol Cell Biol 18, 1946-55.
  8. Ribar, T.J. et al. (2000) J Neurosci 20, RC107.
  9. Tan, Y. et al. (1996) EMBO J 15, 4629-42.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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