Product Pathways - PI3K / Akt Signaling
MAGI3-Akt3 Fusion Protein (D4D3P) Rabbit mAb #48086
|48086S||100 µl ( 10 western blots )||￥3,100.00 现货查询||购买询价|
|48086||carrier free & custom formulation / quantity||email request|
|W||1:1000||Human,||Transfected Only||135||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),
Specificity / Sensitivity
MAGI3-Akt3 Fusion Protein (D4D3P) Rabbit mAb recognizes transfected MAGI3-Akt3 fusion protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the fusion site of MAGI3-Akt3 fusion protein.
Confocal immunofluorescent analysis of MCF 10A cells stably transfected with MAGI3/Akt3 (left) or with a control vector (right), using MAGI3-Akt3 Fusion Protein (D4D3P) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
MAGI3-Akt3 fusion was first detected in triple-negative breast cancer tissues in a sequence analysis research study (1). Rearrangement occurs between the genes MAGI3 (membrane-associated guanylate kinase, WW and PDZ domain containing 3) on chromosome 1p and Akt3 on chromosome 1q, leading to a translocation from intron 9 in MAGI3 to intron1 of Akt3. This rearrangement yields an in-frame fusion gene that translates into a MAGI3-Akt3 fusion protein. Overexpression studies show that MAGI3-Akt3 fusion protein leads to the constitutive activation of Akt kinase (1). Further research studies demonstrate that this fusion is not a recurrent event in triple-negative breast cancer (2,3).
- Banerji, S. et al. (2012) Nature 486, 405-9.
- Mosquera, J.M. et al. (2015) Nature 520, E11-2.
- Pugh, T.J. et al. (2015) Nature 520, E12-4.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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