Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

MAGI3-Akt3 Fusion Protein (D4D3P) Rabbit mAb #48086

No. Size Price
48086S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
48086 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Transfected Only 135 Rabbit IgG
IP 1:50
IF-IC 1:3200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

MAGI3-Akt3 Fusion Protein (D4D3P) Rabbit mAb recognizes transfected MAGI3-Akt3 fusion protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the fusion site of MAGI3-Akt3 fusion protein.

IF-IC

IF-IC

Confocal immunofluorescent analysis of MCF 10A cells stably transfected with MAGI3/Akt3 (left) or with a control vector (right), using MAGI3-Akt3 Fusion Protein (D4D3P) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of extracts from MCF 10A cells, mock-transfected (-) or transfected with human MAGI3/Akt3 (hMAGI3/Akt3; +), using MAGI3-Akt3 Fusion Protein (D4D3P) Rabbit mAb (upper) and β-Actin (13E5) Rabbit mAb #4970 (lower).

Background

MAGI3-Akt3 fusion was first detected in triple-negative breast cancer tissues in a sequence analysis research study (1). Rearrangement occurs between the genes MAGI3 (membrane-associated guanylate kinase, WW and PDZ domain containing 3) on chromosome 1p and Akt3 on chromosome 1q, leading to a translocation from intron 9 in MAGI3 to intron1 of Akt3. This rearrangement yields an in-frame fusion gene that translates into a MAGI3-Akt3 fusion protein. Overexpression studies show that MAGI3-Akt3 fusion protein leads to the constitutive activation of Akt kinase (1). Further research studies demonstrate that this fusion is not a recurrent event in triple-negative breast cancer (2,3).

  1. Banerji, S. et al. (2012) Nature 486, 405-9.
  2. Mosquera, J.M. et al. (2015) Nature 520, E11-2.
  3. Pugh, T.J. et al. (2015) Nature 520, E12-4.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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