Cell Signaling Technology

Product Pathways - DNA Damage

Phospho-SMC1 (Ser957) (5D11G5) Mouse mAb #4805

SMC   SMC 1   SMC-1   SMCI   structural maintenance of chromosome 1  

No. Size Price
4805S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
4805 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 145 Mouse IgG1

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Bovine,

Specificity / Sensitivity

Phospho-SMC1 (Ser957) (5D11G5) Mouse Monoclonal Antibody detects endogenous levels of SMC1 only when phosphorylated at serine 957. This antibody may also recognize phosphorylated human DNA-PKcs (450kDa). Phospho-SMC1 (Ser957) (5D11G5) Mouse Monoclonal Antibody 能够检测内源性957位丝氨酸磷酸化的SMC1蛋白水平。该抗体也可识别磷酸化的人DNA-PKcs (450kDa)。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser957 of human SMC1. 该单克隆抗体是由合成的针对人SMC1的957位丝氨酸的磷酸化肽段免疫动物生产的。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 and NIH/3T3 cells, untreated or UV-treated, using Phospho-SMC1 (Ser957) (5D11G5) Mouse Monoclonal Antibody (upper), SMC1 Antibody #4802 (middle), and DNA-PK Antibody #4602 (lower). Western blot方法检测293 和NIH/3T3 细胞提取物,未处理或者紫外处理,使用的抗体为Phospho-SMC1 (Ser957) (5D11G5) Mouse Monoclonal Antibody (上), SMC1 Antibody #4802 (中)和DNA-PK Antibody #4602 (下)。


Structural maintenance of chromosomes 1 (SMC1) protein is a chromosomal protein member of the cohesin complex that enables sister chromatid cohesion and plays a role in DNA repair (1,2). ATM/NBS1-dependent phosphorylation of SMC1 occurs at Ser957 and Ser966 in response to ionizing radiation (IR) as part of the intra-S-phase DNA damage checkpoint (3). SMC1 phosphorylation is ATM-independent in cells subjected to other forms of DNA damage, including UV light and hydroxyurea treatment (4). While phosphorylation of SMC1 is required for activation of the IR-induced intra-S-phase checkpoint, the precise mechanism is not well understood and may involve a conformational change that affects SMC1-SMC3 interaction (3). 染色体结构维持蛋白1(SMC1)是黏连蛋白复合体的一个染色体蛋白成员,该复合体促进姐妹染色单体结合并在DNA修复中起着重要作用(1,2)。作为S期内部DNA损伤检验点的一部分,ATM/NBS1-依赖的SMC1磷酸化发生在第957位和966位丝氨酸以应答离子辐射(IR) (3)。在经历其他形式的DNA损伤包括紫外线和羟基脲处理的细胞中,SMC1的磷酸化是ATM-非依赖的(4)。尽管SMC1的磷酸化是IR诱导的内S期检验点激活所必需的,但精确的机制尚未阐明且有可能涉及影响SMC1-SMC3相互作用的构象变化(3)。

  1. Michaelis, C. et al. (1997) Cell 91, 35-45.
  2. Sjögren, C. and Nasmyth, K. (2001) Curr Biol 11, 991-5.
  3. Yazdi, P.T. et al. (2002) Genes Dev 16, 571-82.
  4. Kim, S.T. et al. (2002) Genes Dev 16, 560-70.

Application References

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