Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

Nucleomethylin Antibody #4789

Cerebral protein 1   hucep-1   KIAA0409   ribosomal RNA-processing protein 8   rrp8  

No. Size Price
4789S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
4789 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 58 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

This antibody detects endogenous levels of total Nucleomethylin protein.


Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of the human Nucleomethylin protein. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Nucleomethylin Antibody.

使用Nucleomethylin Antibody,免疫印迹(Western blot)分析不同细胞中Nucleomethylin的蛋白水平。


Nucleomethylin (NML), also known as ribosomal RNA-processing protein 8 (RRP8) and human cerebral protein 1 (Hucep-1), is a nucleolar protein (1,2). NML interacts with the histone de-acetylase protein SirT1 and histone methyl-transferase protein SUV39H1 to form the energy-dependent nucleolar silencing complex (eNoSC) that regulates ribosomal RNA (rRNA) transcription in response to changes in the energy state of the cell (2). As energy levels in the cell decrease due to caloric restriction, eNoSC binds to rRNA genes and represses transcription by SirT1-mediated de-acetylation of histones and SUV39H1-mediated methylation of histone H3 on Lys9. NML binds to di-methylated Lys9 of histone H3 and likely functions in the recruitment and spreading of eNoSC along the rRNA genes (2). NML also contains a methyltransferases-like domain, which binds to S-adenosyl-methionine (SAM) and is required for eNoSC function (2). By limiting the transcription of rRNA genes during caloric restriction, eNoSC promotes the restoration of energy balance and protects cells from energy-dependent apoptosis.

Nucleomethylin (NML)也称为ribosomal RNA-processing protein 8 (RRP8)和human cerebral protein 1 (Hucep-1),它是一个核仁的蛋白(1,2)。NML与组蛋白去乙酰酶SirT1和组蛋白甲基转移酶SUV39H1相互作用从而形成energy-dependent nucleolar silencing complex (eNoSC),该复合物在应对细胞内能量水平改变时能够调节ribosomal RNA (rRNA)转录(2)。因为细胞内能量水平的减少由于热量的限制,eNoSC可结合到rRNA基因,并且通过SirT1介导组蛋白去乙酰化和SUV39H1介导histone H3蛋白Lys9位点的甲基化从而抑制转录。NML结合到Lys9位点双甲基化的histone H3,并且很可能功能是沿着rRNA基因招募和传播eNoSC(2)。NML也包含一个甲基转移酶样结构域,这能结合到S-adenosyl-methionine (SAM),并且对eNoSC功能是需要的(2)。在热量限制期间通过限定 rRNA基因的转录,eNoSC促进能量平衡的恢复和保护细胞免受能量依赖的凋亡。

  1. Andersen, J.S. et al. (2005) Nature 433, 77-83.
  2. Murayama, A. et al. (2008) Cell 133, 627-39.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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