Cell Signaling Technology

Product Pathways - Protein Stability

Ubc9 (D26F2) XP® Rabbit mAb #4786

p18   SUMO-protein ligase   UBCE9   UBE2I   Ubiquitin carrier protein 9   Ubiquitin carrier protein I   Ubiquitin-conjugating enzyme E2 I   Ubiquitin-protein ligase I  

No. Size Price
4786S 100 µl ( 10 western blots ) ¥3,580.00 现货查询 购买询价
4786T 20 µl ( 2 western blots ) ¥1,400.00 现货查询 购买询价
4786 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 17 Rabbit IgG
IF-IC 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Ubc9 (D26F) XP® 兔单抗识别Ubc9总蛋白。

Source / Purification

Ubc9 (D26F) XP® 兔单抗是通过合成人Ubc9蛋白Ile125邻近的氨基酸残基序列一致的肽段,免疫动物获得。 Western blot analysis of extracts from various cell lines using Ubc9 (D26F2) XP® Rabbit mAb. 使用Ubc9 (D26F2) XP® 兔单抗对不同细胞裂解物进行免疫印迹分析的结果。 Confocal immunofluorescent analysis of A172 cells using Ubc9 (D26F2) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). 使用UBC9(D26F2)XP® 兔单抗检测A172细胞共聚焦免疫荧光分析结果(绿色), DY-554 phalloidin对微丝进行标记(红色)。

IF-IC

IF-IC

Confocal immunofluorescent analysis of A172 cells using Ubc9 (D26F2) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Ubc9 (D26F2) XP® Rabbit mAb.

Background

SUMO-1偶联过程与泛素化修饰和其他翻译后修饰过程相似(1),SUMO-1通过泛素结合酶E1、E2和E3的协同作用而偶联于目标蛋白(2)。Ubc9 (或ube2M)是一个由158氨基酸构成的非常保守的SUMO-1偶联酶(3)。Ubc9通过其SUMO-1-CS (共有序列)结构域结合到靶蛋白上,并通过保守的氨基端结构域与SUMO分子相互作用(3,4)。 定位于核内和核孔的Ubc9可以催化底物蛋白的sumo化修饰并调节靶蛋白的核质转运和转录活性(5,6)。 Ubc9靶蛋白包括RAD51、RAD52、p53和c-Jun,调节细胞周期、DNA损伤和p53介导的其他过程(7)。

  1. Geiss-Friedlander, R. and Melchior, F. (2007) Nat Rev Mol Cell Biol 8, 947-56.
  2. Tatham, M.H. et al. (2003) Biochemistry 42, 3168-79.
  3. Sampson, D.A. et al. (2001) J Biol Chem 276, 21664-9.
  4. Liu, Q. et al. (1999) J Biol Chem 274, 16979-87.
  5. Lee, G.W. et al. (1998) J Biol Chem 273, 6503-7.
  6. Pichler, A. and Melchior, F. (2002) Traffic 3, 381-7.
  7. Mo, Y.Y. and Moschos, S.J. (2005) Expert Opin Ther Targets 9, 1203-16.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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