Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-HER3/ErbB3 (Tyr1222) (50C2) Rabbit mAb #4784

erb b3   erbB-3   HER3   receptor tyrosine-protein kinase erbB-3   v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)  

No. Size Price
4784L 300 µl ( 30 western blots ) ¥9,325.00 现货查询 购买询价
4784S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
4784 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 185 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-HER3/ErbB3 (Tyr1222) (50C2) Rabbit mAb detects endogenous HER3/ErbB3 proteins only when phosphorylated at tyrosine 1222. The antibody does not cross-react with other tyrosine phosphorylated receptor tyrosine kinases.

磷酸化HER3/ErbB3 (Tyr1222) (50C2) 兔单抗仅在Tyr1222被磷酸化后才能检测到内源性HER3/ErbB3的存在。本抗体不并与其它磷酸化的酪氨酸激酶受体发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1222 of human HER3/ErbB3.

单克隆抗体通过用多肽免疫动物得到,该磷酸化多肽是根据人的HER3/ErbB3蛋白yr1222附近的氨基酸序列合成的。

Western Blotting

Western Blotting

Western blot analysis of MCF-7 and T47D cell lysates untreated or stimulated with Neuregulin (100 ng/ml) for 15 min, using Phospho-HER3/ErbB3 (Tyr1222) (50C2) Rabbit mAb (upper) and HER3/ErbB3 (1B2) Rabbit mAb #4754 (lower). western blot方法检测如下处理的MCF-7 和T47D细胞提取物:未处理和用Neuregulin (100ng/ml)处理15分钟。使用抗体为:Phospho-HER3/ErbB3 (Tyr1222) (50C2) Rabbit mAb (上)或HER3/ErbB3 (1B2) Rabbit mAb #4754 (下)。

Background

HER3/ErbB3 is a member of the ErbB receptor protein tyrosine kinase family, but lacks tyrosine kinase activity. Tyrosine phosphorylation of ErbB3 depends on its association with other ErbB tyrosine kinases. Upon ligand binding, heterodimers form between ErbB3 and other ErbB proteins and ErbB3 is phosphorylated on tyrosine residues by the activated ErbB kinase (1,2). There are at least 9 potential tyrosine phosphorylation sites in the carboxy-terminal tail of ErbB3. These sites serve as consensus binding sites for signal transducing proteins, including Src family members, Grb2, and the p85 subunit of PI3 kinase, which mediate ErbB downstream signaling (3). Both Tyr1222 and Tyr1289 of ErbB3 reside within a YXXM motif and participate in signaling to PI3 kinase (4). 
 ErbB3 is highly expressed in many cancer cells (5) and activation of the ErbB3/PI3K pathway is correlated with malignant phenotypes of adenocarcinomas (6). In tumor development, ErbB3 may function as an oncogenic unit together with other ErbB members (e.g. ErbB2 requires ErbB3 to drive breast tumor cell proliferation) (7). Thus, inhibiting interaction between ErbB3 and ErbB tyrosine kinases has become a novel strategy for anti-tumor therapy.

HER3/ErbB3是ErbB酪氨酸激酶受体蛋白家族的成员之一,但却没有酪氨酸激酶活性。ErbB3的酪氨酸位点磷酸化依赖于其它ErbB酪氨酸激酶。与配体结合后,ErbB3与其它ErbB蛋白形成异二聚体,并且其酪氨酸位点能够被激活的ErbB激酶磷酸化(1,2)。在ErbB3的羧基端至少有9个潜在的酪氨酸磷酸化位点。这些位点都是信号转导蛋白一致的结合位点,这些蛋白包括Src家族的分子,Grb2, 和PI3K激酶的p85亚基,它们都能参与ErbB的下游信号调节(3)。ErbB3 的Tyr1222和Tyr1289残基都位于一个YXXM基序上并参与PI3K激酶的信号传递(4)。ErbB3高表达于很多癌细胞中(5),而ErbB3/PI3K通路的激活也和许多腺癌的恶性特征相关(6)。在肿瘤发展过程中,ErbB3可能和ErbB家族的其它成员(如,ErbB2需要和ErbB3一起促进乳腺癌细胞的增殖)作为一个原癌基因单位一起发挥功能(7)。因此,抑制ErbB3和ErbB酪氨酸激酶的相互作用已经成为抗癌治疗中的一个新策略。

  1. Yarden, Y. and Sliwkowski, M.X. (2001) Nat Rev Mol Cell Biol 2, 127-37.
  2. Guy, P.M. et al. (1994) Proc Natl Acad Sci U S A 91, 8132-6.
  3. Songyang, Z. et al. (1993) Cell 72, 767-78.
  4. Kim, H.H. et al. (1994) J Biol Chem 269, 24747-55.
  5. Sithanandam, G. et al. (2003) Carcinogenesis 24, 1581-92.
  6. Kobayashi, M. et al. (2003) Oncogene 22, 1294-301.
  7. Holbro, T. et al. (2003) Proc Natl Acad Sci U S A 100, 8933-8.

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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