Cell Signaling Technology

Product Pathways - Apoptosis

Lamin A/C (4C11) Mouse mAb #4777

lamina/c   laminc   sc-20680   sc-376248   sc-6215  

No. Size Price
4777S 100 µl ( 20 western blots ) ¥3,100.00 现货查询 购买询价
4777T 20 µl ( 2 western blots ) ¥1,200.00 现货查询 购买询价
4777 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:2000 Human,Mouse,Rat,Monkey, Endogenous 74 (Lamin A), 63 (Lamin C) Mouse IgG2a
IP 1:50
IHC-P 1:200
F 1:200
IF-F 1:100
IF-IC 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry, IF-F=Immunofluorescence (Frozen), IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Lamin A/C (4C11) Mouse mAb detects endogenous levels of lamin A and lamin C proteins. It also reacts with the larger fragments of lamin A (50 kDa) and lamin C (41 kDa) produced by caspase cleavage during apoptosis. This antibody does not cross-react with lamins B1 and B2.

Lamin A/C (4C11) Mouse mAb鼠单抗能够检测内源水平的lamin A 和 C 蛋白。它也能检测在凋亡过程中被caspase剪切的大片段lamin A (50 kDa)和lamin C (41 kDa) 。此抗体不与lamins B1和B2交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant fragment of human lamin A protein.

单克隆抗体是采用人lamin A 蛋白的重组片段免疫动物而获得。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Lamin A/C (4C11) Mouse mAb.

Western免疫印迹分析各种细胞的细胞提取液,用的抗体是Lamin A/C (4C11) Mouse mAb 鼠单抗。

Western Blotting

Western Blotting

Western blot analysis of extracts from THP-1 cells, untreated or treated with cycloheximide (CHX, 10 μg/ml, overnight) followed by TNF-α #8902 (20 ng/ml, 4 hours), using Lamin A/C (4C11) Mouse mAb.

Western免疫印迹分析THP-1细胞的细胞提取液,未处理和cycloheximide (CHX, 10 μg/ml, 过夜)处理(随后用TNF-α #8902 (20 ng/ml, 4 小时)),使用 Lamin A/C (4C11) Mouse mAb 鼠单抗。

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using Lamin A/C (4C11) Mouse mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).

共聚焦免疫荧光分析HeLa细胞,所用抗体为Lamin A/C (4C11) Mouse mAb (绿色)鼠单抗。肌动蛋白微丝用 DY-554 phalloidin (红色)标记。

IF-F

IF-F

Immunofluorescent analysis of normal rat brain using Lamin A/C (4C11) Mouse mAb (green) and MAP2 Antibody #4542 (red).

共聚焦免疫荧光分析正常的大鼠脑组织,所用抗体为Lamin A/C (4C11) Mouse mAb 鼠单抗(绿色)和MAP2 Antibody #4542(红色)。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells using Lamin A/C (4C11) Mouse mAb (blue) compared to a nonspecific negative control antibody (red).

流式细胞仪分析HeLa 细胞,所用抗体为Lamin A/C (4C11) Mouse mAb 鼠单抗(蓝色) ,用非特异性的阴性对照抗体(红色)做对比。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Lamin A/C (4C11) Mouse mAb.

免疫组织化学染色分析石蜡包埋人结肠癌组织,所用抗体为 Lamin A/C (4C11) Mouse mAb 鼠抗体。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Lamin A/C (4C11) Mouse mAb.

免疫组织化学染色分析石蜡包埋人乳腺癌组织,所用抗体为 Lamin A/C (4C11) Mouse mAb 鼠单抗。

Background

Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication and chromatin organization (1-3). Lamin A/C is cleaved by caspase-6 and serves as a marker for caspase-6 activation. During apoptosis, lamin A/C is specifically cleaved into a large (41-50 kDa) and a small (28 kDa) fragment (3,4). The cleavage of lamins results in nuclear disregulation and cell death (5,6).

Lamins是核膜的结构组分,对维持正常的细胞功能非常重要,比如细胞周期调控、DNA修复和染色质组装(1-3)。Lamin A/C能够被caspase-6切割,可以作为caspase-6激活的标志。在凋亡过程中,Lamin A/C能够被特异地剪切成大片段(41-50 kDa)和小片段 (28 kDa)(3,4)。Lamin的剪切会引起细胞核的失调和细胞死亡(5,6)。

  1. Gruenbaum, Y. et al. (2000) J Struct Biol 129, 313-23.
  2. Yabuki, M. et al. (1999) Physiol Chem Phys Med NMR 31, 77-84.
  3. Goldberg, M. et al. (1999) Crit Rev Eukaryot Gene Expr 9, 285-93.
  4. Orth, K. et al. (1996) J Biol Chem 271, 16443-6.
  5. Oberhammer, F.A. et al. (1994) J Cell Biol 126, 827-37.
  6. Rao, L. et al. (1996) J Cell Biol 135, 1441-55.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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