Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

Acetyl-CBP (Lys1535)/p300 (Lys1499) Antibody #4771

acetyltransferase   CBP   HAT   p300  

No. Size Price
4771S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
4771T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
4771 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Monkey, Endogenous 300 Rabbit
IP 1:50
ChIP 1:25

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, ChIP=Chromatin IP,


Species predicted to react based on 100% sequence homology: Rat,

Specificity / Sensitivity

Acetyl-CBP (Lys1535)/p300 (Lys1499) Antibody detects endogenous levels of CBP or p300 only when acetylated at lysine 1535 or lysine 1499, respectively.

Acetyl-CBP (Lys1535)/p300 (Lys1499) Antibody分别检测仅在lysine 1535或lysine 1499位点乙酰化的内源性CBP或p300蛋白水平(亚型1)。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic acetylated peptide corresponding to residues surrounding Lys1535 of human CBP. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of hypo- or hyper-acetylated recombinant p300 HAT domains, either wild-type or K1499R mutant, using Acetyl-CBP (Lys1535)/p300 (Lys1499) Antibody (upper). Also shown in the corresponding coomassie stained SDS-PAGE gel (lower). (Details are described in Thompson, P.A. et al. (2004) Nat. Struct. Mol. Biol. 11, 308-315.)

使用Acetyl-CBP (Lys1535)/p300 (Lys1499) Antibody (上图),免疫印迹(Western blot)分析hypo- or hyper-乙酰化的重组p300 HAT结构域,wild-type 或K1499R突变。 下图是相应的考马斯亮蓝染色的SDS-PAGE胶的结果。(Details are described in Thompson, P.A. et al. (2004) Nat. Struct. Mol. Biol. 11, 308-315.)

Western Blotting

Western Blotting

Western blot analysis of extracts from A431, NIH/3T3, COS and PC12 cells, using Acetyl-CBP (Lys1535)/p300 (Lys1499) Antibody.

使用Acetyl-CBP (Lys1535)/p300 (Lys1499) Antibody,免疫印迹(Western blot)分析A431、NIH/3T3、COS和PC12细胞中Acetyl-CBP (Lys1535)/p300 (Lys1499)的蛋白水平。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 293 cells treated with Forskolin #3828 (30uM) and either 20 μl of Acetyl-CBP (Lys1535)/p300 (Lys1499) Antibody or 2 μl of Normal Rabbit IgG #2729, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human ALS2 exon 1 primers, SimpleChIP® Human NR4A3 Promoter Primers #4829, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

使用SimpleChIP®Enzymatic Chromatin IP Kit (Magnetic Beads) #9003,用Forskolin #3828 (30uM)处理过的4 x 106 293细胞的交联染色质以及20 µl Acetyl-CBP (Lys1535)/p300 (Lys1499) Antibody或2 µl Normal Rabbit IgG #2729进行染色质免疫沉淀实验。使用human ALS2 exon 1 primers, SimpleChIP® Human NR4A3 Promoter Primers #4829和SimpleChIP® Human α Satellite Repeat Primers #4486,浓缩的DNA通过real-time PCR定量。在每个样品中免疫沉淀DNA的数量被当做一个相对于总input chromatin的数量的信号,这相当于一。


CBP (CREB-binding protein) and p300 are highly conserved and functionally related transcriptional co-activators that associate with transcriptional regulators and signaling molecules, integrating multiple signal transduction pathways with the transcriptional machinery (1,2). CBP/p300 also contain histone acetyltransferase (HAT) activity, allowing them to acetylate histones and other proteins (2). Phosphorylation of p300 at Ser89 by PKC represses its transciptional acitivity, and phosphorylation at the same site by AMPK disrupts the association of p300 with nuclear receptors (3,4). Ser1834 phosphorylation of p300 by Akt disrupts its association with C/EBPβ (5). Growth factors induce phosphorylation of CBP at Ser437, which is required for CBP recruitment to the transcription complex (6). CaM kinase IV phosphorylates CBP at Ser302, which is required for CBP-dependent transcriptional activation in the CNS (7). The role of acetylation of CBP/p300 is of particular interest (2,8). Acetylation of Lys1499 of p300 has been demonstrated to enhance its HAT activity and affect a wide variety of signaling events (9).

CBP (CREB-binding protein)和p300蛋白是高度保守的并且具有与转录共激活因子有关联的功能,它是与转录调节因子和信号分子有关联,使用转录分子去整合多种信号转导通路(1,2)。CBP/p300也包含组蛋白乙酰转移酶(HAT)活性,它允许它们使组蛋白或其它蛋白发生乙酰化(2)。通过PKC使p300蛋白在Ser89位点的磷酸化抑制它的转录活性,并且通过AMPK使相同位点的磷酸化干扰了细胞核受体与p300蛋白的联系(3,4)。Akt使p300蛋白Ser1834位点的磷酸化干扰它与C/EBPβ的联系(5)。生长因子可诱导CBP在Ser437位点磷酸化,这对于CBP被招募到转录复合物上时需要的(6)。CaM激酶IV 使CBP蛋白的Ser302位点磷酸化,这在中枢神经系统中对于CBP依赖的转录活性是需要的(7)。人们对CBP/p300蛋白乙酰化的作用很感兴趣(2,8)。p300蛋白Lys1499位点的乙酰化已经被证明能提高它的HAT活性,并且影响很多信号传导(9)。

  1. Goodman, R.H. and Smolik, S. (2000) Genes Dev 14, 1553-77.
  2. Chan, H.M. and La Thangue, N.B. (2001) J. Cell Sci. 114, 2363-2373.
  3. Yuan, L.W. and Gambee, J.E. (2000) J. Biol. Chem. 275, 40946-40951.
  4. Yang, W. et al. (2001) J. Biol. Chem. 276, 38341-38344.
  5. Guo, S. et al. (2001) J. Biol. Chem. 276, 8516-8523.
  6. Zanger, K. et al. (2001) Mol. Cell 7, 551-558.
  7. Impey, S. et al. (2002) Neuron 34, 235-244.
  8. Yuan, L.W. and Giordano, A. (2002) Oncogene 21, 2253-2260.
  9. Thompson, P.R. et al. (2004) Nat. Struct. Mol. Biol. 11, 308-315.
  10. Stiehl, D.P. et al. (2007) Cancer Res 67, 2256-64.

Application References

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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