Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-HER4/ErbB4 (Tyr1284) (21A9) Rabbit mAb #4757

ERBB4   receptor tyrosine-protein kinase erbB-4   tyrosine kinase-type cell surface receptor HER4   v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian) {ERBB4}  

No. Size Price
4757S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
4757T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
4757 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 180 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Mouse, Rat,

Specificity / Sensitivity

Phospho-HER4/ErbB4 (Tyr1284) (21A9) Rabbit mAb detects endogenous levels of HER4/ErbB4 protein only when phosphorylated on Tyr1284. This antibody does not cross-react with other ErbB family members.

磷酸化HER4/ErbB4 (Tyr1284) (21A9) 兔单抗仅在Tyr1284位点磷酸化时能检测到内源HER4/ErbB4蛋白的表达。本抗体不与其它ErbB家族的蛋白发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1284 of human HER4/ErbB4.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines expressing different activated protein tyrosine kinases, using Phospho-HER4/ErbB4 (Tyr1284) (21A9) Rabbit mAb or Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 (lower). Western blot法检测各种表达不同激活酪氨酸激酶的细胞的提取物,使用抗体为:Phospho-HER4/ErbB4 (Tyr1284) (21A9) Rabbit mAb (上)或Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411(下)。


The HER/ErbB receptor tyrosine kinase family has been implicated in normal development, cardiac function and cancer (1,2). HER4/ErbB4, like other family members, has four ectodomains, a single transmembrane domain and a cytoplasmic tail containing the active tyrosine kinase domain (3). By binding to neuregulins and/or EGF family ligands, ErbB4 forms either a homodimer or heterodimer with other ErbB family members, which results in receptor activation and signaling (3). ErbB4 is ubiquitously expressed with the highest expression occurring in brain and heart. The expression of ErbB4 in breast cancer, pediatric brain cancer and other types of carcinomas has been reported suggesting that ErbB4 expression is involved in both normal tissue development and carcinogenesis (3).


  1. Holbro, T. and Hynes, N.E. (2004) Annu Rev Pharmacol Toxicol 44, 195-217.
  2. Roskoski, R. (2004) Biochem Biophys Res Commun 319, 1-11.
  3. Carpenter, G. (2003) Exp Cell Res 284, 66-77.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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