Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-HER4/ErbB4 (Tyr1284) (21A9) Rabbit mAb #4757

ERBB4   receptor tyrosine-protein kinase erbB-4   tyrosine kinase-type cell surface receptor HER4   v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian) {ERBB4}  

No. Size Price
4757S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
4757T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
4757 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 180 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat,

Specificity / Sensitivity

Phospho-HER4/ErbB4 (Tyr1284) (21A9) Rabbit mAb detects endogenous levels of HER4/ErbB4 protein only when phosphorylated on Tyr1284. This antibody does not cross-react with other ErbB family members.

磷酸化HER4/ErbB4 (Tyr1284) (21A9) 兔单抗仅在Tyr1284位点磷酸化时能检测到内源HER4/ErbB4蛋白的表达。本抗体不与其它ErbB家族的蛋白发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1284 of human HER4/ErbB4.

单克隆抗体通过用多肽免疫动物得到,该磷酸化多肽是根据人的HER4/ErbB4.蛋白Tyr1284附近的氨基酸序列合成的。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines expressing different activated protein tyrosine kinases, using Phospho-HER4/ErbB4 (Tyr1284) (21A9) Rabbit mAb or Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 (lower). Western blot法检测各种表达不同激活酪氨酸激酶的细胞的提取物,使用抗体为:Phospho-HER4/ErbB4 (Tyr1284) (21A9) Rabbit mAb (上)或Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411(下)。

Background

The HER/ErbB receptor tyrosine kinase family has been implicated in normal development, cardiac function and cancer (1,2). HER4/ErbB4, like other family members, has four ectodomains, a single transmembrane domain and a cytoplasmic tail containing the active tyrosine kinase domain (3). By binding to neuregulins and/or EGF family ligands, ErbB4 forms either a homodimer or heterodimer with other ErbB family members, which results in receptor activation and signaling (3). ErbB4 is ubiquitously expressed with the highest expression occurring in brain and heart. The expression of ErbB4 in breast cancer, pediatric brain cancer and other types of carcinomas has been reported suggesting that ErbB4 expression is involved in both normal tissue development and carcinogenesis (3).

研究表明HER/ErbB酪氨酸激酶受体家族在正常发育、心脏功能和肿瘤中发挥作用(1,2)。HER4/ErbB4和家族其它成员一样,有四个胞外功能区,一个跨膜区和一个具有激活酪氨酸激酶活性的胞内区(3)。通过结合神经调节素(NRGs)和/或EGF家族配体,ErbB4形成同源二聚体或与ErbB家族的其它成员形成异二聚体,进而使受体活化进行信号传递(3)。ErbB4普遍高表达于脑和心脏。ErbB4在乳腺癌、小儿脑癌和其它种类肿瘤上的表达和正常组织发育以及肿瘤的发生均有关系(3)。

  1. Holbro, T. and Hynes, N.E. (2004) Annu Rev Pharmacol Toxicol 44, 195-217.
  2. Roskoski, R. (2004) Biochem Biophys Res Commun 319, 1-11.
  3. Carpenter, G. (2003) Exp Cell Res 284, 66-77.

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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