Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

MYST1 (D5T3R) Rabbit mAb #46862

KAT8  

No. Size Price
46862S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
46862 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 53 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

MYST1 (D5T3R) Rabbit mAb recognizes endogenous levels of total MYST1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein surrounding Val80 of human MYST1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using MYST1 (D5T3R) Rabbit mAb.

Background

MYST1, also known as mammalian male absent on the first (MOF) and lysine acetyltransferase 8 (KAT8), is a member of the MYST (MOZ, YBF2, SAS2 and Tip60) family of histone acetyltransferases (1,2). As the catalytic subunit of two different histone acetyltransferase complexes, MSL and NSL, MYST1 is responsible for the majority of histone H4 lysine 16 acetylation in the cell. MYST1 also acetylates p53 on lysine 120 and is important for activation of pro-apoptotic genes (1,2). As a component of the MSL complex, MYST1 associates with MSL1, MSL2L1, and MSL3L1, and specifically acetylates histone H4 on lysine 16 (3-5). As part of the NSL complex, MYST1 associates with the MLL1 histone methyltransferase complex containing MLL1/KMT2A, ASH2L, HCFC1, WDR5 and RBBP5, and shows broader acetyltransferase activity for histone H4 on lysines 5, 8, and 16 (3-5). MYST1 plays a critical role in the regulation of transcription, DNA repair, autophagy, apoptosis, and emybryonic stem cell pluripotency and differentiation (1,2,6). Loss of MYST1 leads to a global reduction in histone H4 lysine 16 acetylation, a common hallmark found in many human cancers. A reduction of MYST1 protein levels and histone H4 lysine 16 acetylation is associated with poor prognosis in breast, renal, colorectal, gastric, and ovarian cancers (1).

  1. Yang, Y. et al. (2014) Front Med 8, 79-83.
  2. Li, X. and Dou, Y. (2010) Epigenetics 5, 185-8.
  3. Dou, Y. et al. (2005) Cell 121, 873-85.
  4. Li, X. et al. (2009) Mol Cell 36, 290-301.
  5. Cai, Y. et al. (2010) J Biol Chem 285, 4268-72.
  6. Füllgrabe, J. et al. (2013) Nature 500, 468-71.

Application References

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