Cell Signaling Technology

Product Pathways - Apoptosis

Caspase-5 (D3G4W) Rabbit mAb #46680

No. Size Price
46680S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
46680 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 50, 44, 35 Rabbit IgG
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Caspase-5 (D3G4W) Rabbit mAb recognizes endogenous levels of total caspase-5 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro154 of human caspase-5 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from THP-1 cells differentiated with TPA #4174 (80 nM, overnight) followed by treatment with LPS #14011 (1 μg/ml, indicated times), using Caspase-5 (D3G4W) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower)

Western Blotting

Western Blotting

Western blot analysis of extracts form 293T cells, mock transfected (-) or transfected with constructs expressing full-length human caspase-4 protein (hCasp4; +) or caspase-5 protein (hCasp5; +), using Caspase-5 (D3G4W) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).

Background

Caspase-5 (Ich-3/ICErelIII/TY) is a member of the caspase family of cysteine proteases that play a key role in the execution of apoptosis and activation of inflammatory cytokines (1-3). Caspase-5 is widely expressed, with highest expression observed in placenta and lung (1). Interferon-γ and LPS regulate expression of caspase-5 (2,4). Members of the caspase-1 subfamily of caspases, which includes caspase-4, -5, and murine caspase-11 and -12, can induce apoptosis when over-expressed and mediate the proteolytic activation of inflammatory cytokines (5). Processing of IL-1β occurs through the activation of an inflammasome complex consisting of caspase-1, caspase-5, Pycard and NALP1 (6). Transcription factor Max, a component of the Myc/Mad/Max network, is cleaved by caspase-5 during Fas-induced apoptosis (7). Several alternative spliced variants of caspase-5 have been identified (8). Frameshift mutations of caspase-5 have been observed in leukemia, lymphoma (9), and colorectal cancers (10).

  1. Munday, N.A. et al. (1995) J Biol Chem 270, 15870-6.
  2. Wang, S. et al. (1996) J Biol Chem 271, 20580-7.
  3. Faucheu, C. et al. (1996) Eur J Biochem 236, 207-13.
  4. Lin, X.Y. et al. (2000) J Biol Chem 275, 39920-6.
  5. Martinon, F. and Tschopp, J. (2007) Cell Death Differ 14, 10-22.
  6. Martinon, F. et al. (2002) Mol Cell 10, 417-26.
  7. Krippner-Heidenreich, A. et al. (2001) Biochem J 358, 705-15.
  8. Eckhart, L. et al. (2006) Biochem Biophys Res Commun 348, 682-8.
  9. Takeuchi, S. et al. (2003) Leuk Res 27, 359-61.
  10. Trojan, J. et al. (2004) Int J Colorectal Dis 19, 538-44.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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