Cell Signaling Technology

Product Pathways - Protein Folding

HSP60 (D6F1) XP® Rabbit mAb (HRP Conjugate) #46611

No. Size Price
46611S 100 µl ( 10 western blots ) ¥4,264.00 现货查询 购买询价
46611 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Hamster,Monkey,Xenopus,Zebrafish,Bovine,Pig, Endogenous 60 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Chicken, Dog, Horse,

Specificity / Sensitivity

HSP60 (D6F1) XP® Rabbit mAb (HRP Conjugate) recognizes endogenous levels of total HSP60 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Trp68 of human HSP60 protein.


This Cell Signaling Technology antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated HSP60 (D6F1) XP® Rabbit mAb #12165.

Western Blotting

Western Blotting

Western blot analysis of of extracts from HeLa and COS-7 cell lines using HSP60 (D6F1) XP® Rabbit mAb (HRP Conjugate).


In both prokaryotic and eukaryotic cells the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones (1-3). HSP60 has primarily been known as a mitochondrial protein that is important for folding key proteins after import into the mitochondria (4). Research studies have shown that a significant amount of HSP60 is also present in the cytosol of many cells, and that it is induced by stress, inflammatory and immune responses, and autoantibodies correlated with Alzheimer's, coronary artery diseases, MS, and diabetes (5-8).

  1. Hartl, F.U. (1996) Nature 381, 571-579.
  2. Bukau, B. and Horwich, A.L. (1998) Cell 92, 351-366.
  3. Hartl, F.U. and Hayer-Hartl, M. (2002) Science 295, 1852-1858.
  4. Jindal, S. et al. (1989) Mol. Cell Biol. 9, 2279-2283.
  5. Itoh, H. et al. (2002) Eur. J. Biochem. 269, 5931-5938.
  6. Gupta, S. and Knowlton, A.A. J. Cell Mol. Med. 9, 51-58.
  7. Deocaris, C.C. et al. (2006) Cell Stress Chaperones 11, 116-128.
  8. Lai, H.C. et al. (2007) Am. J. Physiol. Endocrinol. Metab. 292, E292-E297.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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