Cell Signaling Technology

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Anti-Blue (2D2F11) Mouse mAb (HRP Conjugate) #46387

Blue   Ladder   marker   molecular weight marker   pre stained   pre-stained   prestained   protein standard   Remazol   secondary  

No. Size Price
46387S 100 µl ( 10 western blots ) ¥3,986.00 现货查询 购买询价
46387 carrier free & custom formulation / quantityemail request
Applications Species-Reactivity Sensitivity MW (kDa) Isotype
W Recombinant Mouse

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Anti-Blue (2D2F11) Mouse mAb (HRP Conjugate) recognizes remazol blue dye attached to proteins.

Description

Anti-Blue (2D2F11) Mouse mAb is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. This product has been optimized to detect proteins labeled with remazol blue.

Western Blotting

Western Blotting

Titration of Prestained Protein Marker, Broad Range (11-190 kDa) #13953 on a 4-20% Tris-glycine gradient SDS-polyacrylamide gel (left). Western Blot analysis of #13953 using Anti-Blue (2D2F11) Mouse mAb (HRP Conjugate) (right).

Western Blotting

Western Blotting

Titration of Pierce™ Prestained Protein MW markers [ThermoFisher #26612] on a 4-20% Tris-glycine gradient SDS-polyacrylamide gel (left). Western Blot analysis of Pierce™ Prestained Protein MW markers [ThermoFisher #26612] using Anti-Blue (2D2F11) Mouse mAb (HRP Conjugate) (right).

Western Blotting

Western Blotting

Titration of PageRuler™ Prestained Protein ladder, 10 to 180 kDa [ThermoFisher #26616] on a 4-20% Tris-glycine gradient SDS-polyacrylamide gel (left). Western Blot analysis of PageRuler™ Prestained Protein ladder, 10 to 180 kDa [ThermoFisher #26616] using Anti-Blue (2D2F11) Mouse mAb (HRP Conjugate) (right).

Background

Chemiluminescence systems have emerged as the best all-around method for western blot detection. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives, and because results are generated on film, it is possible to record and store data permanently. Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP conjugates have a very high turnover rate, yielding good sensitivity with short reaction times.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

Thermo Fisher is a registered trademark of Thermo Fisher Scientific, Inc.

PageRuler is a trademark of Thermo Fisher Scientifc, Inc.

Pierce is a trademark of Thermo Fisher Scientific, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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