Product Pathways - Cytoskeletal Signaling
Cavin-1 (D8C1D) Rabbit mAb #46379
|46379S||100 µl ( 10 western blots )||￥3,100.00 现货查询||购买询价|
|46379||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),
Specificity / Sensitivity
Cavin-1 (D8C1D) Rabbit mAb recognizes endogenous levels of total cavin-1 protein. Based on the sequence of the immunogenic peptide, this antibody is not expected to cross-react with other cavin family members.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala353 of human cavin-1 protein.
Confocal immunofluorescent analysis of ACHN (left) and LNCaP (right) cells using Cavin-1 (D8C1D) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Western blot analysis of extracts from various cell lines using Cavin-1 (D8C1D) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Caveolae ("little caves") are 60-80 nm pits representing specialized plasma membrane domains in many cell types. The principal protein component of caveolae is caveolin, a small integral membrane protein composed of three family members, including the widely expressed caveolin-1 and -2, and the muscle-specific caveolin-3 (1). Caveolin proteins are required for caveolae formation and serve as scaffolding proteins for the recruitment of signaling proteins. Research studies in cavelolin-deficient mice implicate cavelolin proteins in several pathologies, including diabetes, cancer, cardiovascular diseases, atherosclerosis, pulmonary disease, and muscular dystrophies (2).
The cavin proteins (cavin-1, -2, -3, and -4 in mammals) are a family of caveolae-associated integral membrane proteins involved in the biogenesis and stability of caveolae. Cavin proteins form homo- or hetero-oligomers whose composition is tissue-specific, which may confer distinct functions of caveolae in various tissues (3). Cavin-1 (PTRF), which is widely expressed, is required for caveolae formation and is thought to play roles in lipid metabolism, adipocyte differentiation, and IGF-1 receptor signaling (4-6). Research studies involving prostate cancer suggest that expression of cavin-1 is related to tumor progression and angiogenesis/lymphangiogenesis (7-8).
- Bastiani, M. and Parton, R.G. (2010) J Cell Sci 123, 3831-6.
- Cohen, A.W. et al. (2004) Physiol Rev 84, 1341-79.
- Kovtun, O. et al. (2015) J Cell Sci 128, 1269-78.
- Ding, S.Y. et al. (2014) J Biol Chem 289, 8473-83.
- Perez-Diaz, S. et al. (2014) FASEB J 28, 3769-79.
- Hamoudane, M. et al. (2013) J Endocrinol Invest 36, 204-8.
- Nassar, Z.D. et al. (2013) Nat Rev Urol 10, 529-36.
- Nassar, Z.D. et al. (2013) Oncotarget 4, 1844-55.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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For Research Use Only. Not For Use In Diagnostic Procedures.
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