Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Cavin-1 (D8C1D) Rabbit mAb #46379

No. Size Price
46379S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
46379 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 50 Rabbit IgG
IP 1:50
IF-IC 1:800

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Cavin-1 (D8C1D) Rabbit mAb recognizes endogenous levels of total cavin-1 protein. Based on the sequence of the immunogenic peptide, this antibody is not expected to cross-react with other cavin family members.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala353 of human cavin-1 protein.

IF-IC

IF-IC

Confocal immunofluorescent analysis of ACHN (left) and LNCaP (right) cells using Cavin-1 (D8C1D) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Cavin-1 (D8C1D) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

IP

IP

Immunoprecipitation of Cavin-1 from ACHN cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Cavin-1 (D8C1D) Rabbit mAb. Western blot was performed using Cavin-1 (D8C1D) Rabbit mAb. A light chain-specific secondary antibody was used.

Background

Caveolae ("little caves") are 60-80 nm pits representing specialized plasma membrane domains in many cell types. The principal protein component of caveolae is caveolin, a small integral membrane protein composed of three family members, including the widely expressed caveolin-1 and -2, and the muscle-specific caveolin-3 (1). Caveolin proteins are required for caveolae formation and serve as scaffolding proteins for the recruitment of signaling proteins. Research studies in cavelolin-deficient mice implicate cavelolin proteins in several pathologies, including diabetes, cancer, cardiovascular diseases, atherosclerosis, pulmonary disease, and muscular dystrophies (2).

The cavin proteins (cavin-1, -2, -3, and -4 in mammals) are a family of caveolae-associated integral membrane proteins involved in the biogenesis and stability of caveolae. Cavin proteins form homo- or hetero-oligomers whose composition is tissue-specific, which may confer distinct functions of caveolae in various tissues (3). Cavin-1 (PTRF), which is widely expressed, is required for caveolae formation and is thought to play roles in lipid metabolism, adipocyte differentiation, and IGF-1 receptor signaling (4-6). Research studies involving prostate cancer suggest that expression of cavin-1 is related to tumor progression and angiogenesis/lymphangiogenesis (7-8).

  1. Bastiani, M. and Parton, R.G. (2010) J Cell Sci 123, 3831-6.
  2. Cohen, A.W. et al. (2004) Physiol Rev 84, 1341-79.
  3. Kovtun, O. et al. (2015) J Cell Sci 128, 1269-78.
  4. Ding, S.Y. et al. (2014) J Biol Chem 289, 8473-83.
  5. Perez-Diaz, S. et al. (2014) FASEB J 28, 3769-79.
  6. Hamoudane, M. et al. (2013) J Endocrinol Invest 36, 204-8.
  7. Nassar, Z.D. et al. (2013) Nat Rev Urol 10, 529-36.
  8. Nassar, Z.D. et al. (2013) Oncotarget 4, 1844-55.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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