Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Cavin-1 (D8C1D) Rabbit mAb #46379

No. Size Price
46379S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
46379 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 50 Rabbit IgG
IP 1:50
IF-IC 1:800

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Cavin-1 (D8C1D) Rabbit mAb recognizes endogenous levels of total cavin-1 protein. Based on the sequence of the immunogenic peptide, this antibody is not expected to cross-react with other cavin family members.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala353 of human cavin-1 protein.



Confocal immunofluorescent analysis of ACHN (left) and LNCaP (right) cells using Cavin-1 (D8C1D) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Cavin-1 (D8C1D) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).



Immunoprecipitation of Cavin-1 from ACHN cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Cavin-1 (D8C1D) Rabbit mAb. Western blot was performed using Cavin-1 (D8C1D) Rabbit mAb. A light chain-specific secondary antibody was used.


Caveolae ("little caves") are 60-80 nm pits representing specialized plasma membrane domains in many cell types. The principal protein component of caveolae is caveolin, a small integral membrane protein composed of three family members, including the widely expressed caveolin-1 and -2, and the muscle-specific caveolin-3 (1). Caveolin proteins are required for caveolae formation and serve as scaffolding proteins for the recruitment of signaling proteins. Research studies in cavelolin-deficient mice implicate cavelolin proteins in several pathologies, including diabetes, cancer, cardiovascular diseases, atherosclerosis, pulmonary disease, and muscular dystrophies (2).

The cavin proteins (cavin-1, -2, -3, and -4 in mammals) are a family of caveolae-associated integral membrane proteins involved in the biogenesis and stability of caveolae. Cavin proteins form homo- or hetero-oligomers whose composition is tissue-specific, which may confer distinct functions of caveolae in various tissues (3). Cavin-1 (PTRF), which is widely expressed, is required for caveolae formation and is thought to play roles in lipid metabolism, adipocyte differentiation, and IGF-1 receptor signaling (4-6). Research studies involving prostate cancer suggest that expression of cavin-1 is related to tumor progression and angiogenesis/lymphangiogenesis (7-8).

  1. Bastiani, M. and Parton, R.G. (2010) J Cell Sci 123, 3831-6.
  2. Cohen, A.W. et al. (2004) Physiol Rev 84, 1341-79.
  3. Kovtun, O. et al. (2015) J Cell Sci 128, 1269-78.
  4. Ding, S.Y. et al. (2014) J Biol Chem 289, 8473-83.
  5. Perez-Diaz, S. et al. (2014) FASEB J 28, 3769-79.
  6. Hamoudane, M. et al. (2013) J Endocrinol Invest 36, 204-8.
  7. Nassar, Z.D. et al. (2013) Nat Rev Urol 10, 529-36.
  8. Nassar, Z.D. et al. (2013) Oncotarget 4, 1844-55.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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