Cell Signaling Technology

Product Pathways - Metabolism

MPC2 (D4I7G) Rabbit mAb #46141

BR44   Brain protein 44   BRP44   DKFZp564B167   MGC125752   MGC125753   MPC   MPC2   pyruvate carrier   pyruvate carrier 2   sc-134645  

No. Size Price
46141S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
46141 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 14 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

MPC2 (D4I7G) Rabbit mAb recognizes endogenous levels of total MPC2 protein. This antibody does not cross-react with MPC1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn33 of human MPC2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using MPC2 (D4I7G) Rabbit mAb.

Background

The transport of the glycolytic end product pyruvate into mitochondria and the decarboxylation of pyruvate in the citric acid cycle generate energy through oxidative phosphorylation under aerobic conditions (1,2). Two inner mitochondrial membrane proteins, mitochondrial pyruvate carrier 1 (MPC1) and mitochondrial pyruvate carrier 2 (MPC2), form a 150 kDa complex and are essential proteins in the facilitated transport of pyruvate into mitochondria (1,2). Mutations in the corresponding MPC1 gene are associated with deficient pyruvate transport and may result in lactic acidosis, developmental delay, and premature death (2,3). Altered MPC1/MPC2 expression or activity may result in significant metabolic disorders and contribute to the increase in aerobic glycolysis in cancer cells (a.k.a., the Warburg effect) (4).

  1. Herzig, S. et al. (2012) Science 337, 93-6.
  2. Bricker, D.K. et al. (2012) Science 337, 96-100.
  3. Brivet, M. et al. (2003) Mol Genet Metab 78, 186-92.
  4. Gray, L.R. et al. (2014) Cell Mol Life Sci 71, 2577-604.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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