Cell Signaling Technology

Product Pathways - Neuroscience

Doublecortin Antibody #4604

DBCN   DCX   Doublin   Lis-X   Lissencephalin-X   LISX   Neuronal migration protein doublecortin   sc-8066  

No. Size Price
4604S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
4604 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey,D. melanogaster, Endogenous 45 Rabbit
IP 1:50
F 1:100
IF-F 1:400

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry, IF-F=Immunofluorescence (Frozen),

Specificity / Sensitivity

Doublecortin Antibody detects endogenous levels of total doublecortin protein.

Doublecortin Antibody 兔单抗识别内源性的总Doublecortin蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human doublecortin. Antibodies are purified by protein A and peptide affinity chromatography.


Flow Cytometry

Flow Cytometry

Flow cytometric analysis of SK-N-MC cells, using Doublecortin antibody (blue) compared to a nonspecific negative control antibody (red).

流式细胞仪分析SK-N-MC细胞,使用Doublecortin antibody 兔多抗(蓝)和非特异的阴性对照抗体(红)进行标记。



Confocal immunofluorescent analysis of Doublecortin Antibody (red) and GFAP (GA5) Mouse mAb #3670 (green) staining the subventricular zone of the striatum in reperfused ischemic rat brain. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

共聚焦免疫荧光分析缺血再灌注大鼠脑室下纹状体区域,使用Doublecortin Antibody 兔多抗(红色)和GFAP (GA5) Mouse mAb 鼠单抗#3670(绿色)进行标记。蓝色伪彩=DRAQ ®#4084(荧光DNA染料)。

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse and rat brain, using Doublecortin Antibody.

Western blot分析大鼠和小鼠大脑提取物,使用的抗体是Doublecortin Antibody 兔多抗。

Western Blotting

Western Blotting

Western blot analysis of extracts from fetal rat brain and rat brain, using Doublecortin Antibody (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). 


Mutations in Doublecortin cause Lissencephaly (smooth brain), a neuronal migration disorder characterized by epilepsy and mental retardation (1). Doublecortin is a microtubule associated protein that stabilizes and bundles microtubules. A conserved doublecortin domain mediates the interaction with microtubules, and interestingly most missense mutations cluster in this domain (2). Kinases JNK, CDK5 and PKA phosphorylate doublecortin. JNK phosphorylates Thr321, Thr331 and Ser334 while PKA phosphorylates Ser47 and CDK5 phosphorylates Ser297 (3-5). Phosphorylation of Ser297 lowers the affinity of doublecortin to microtubules. Furthermore, mutations of Ser297 result in migration defects (5).


  1. Gleeson, J.G. et al. (1998) Cell 92, 63-72.
  2. Reiner, O. et al. (2004) Cell Cycle 3, 747-51.
  3. Gdalyahu, A. et al. (2004) EMBO J 23, 823-32.
  4. Schaar, B.T. et al. (2004) Neuron 41, 203-13.
  5. Tanaka, T. et al. (2004) Neuron 41, 215-27.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0


我要参与评论 :