Cell Signaling Technology

Product Pathways - Neuroscience

Doublecortin Antibody #4604

DBCN   DCX   Doublin   Lis-X   Lissencephalin-X   LISX   Neuronal migration protein doublecortin   sc-8066  

No. Size Price
4604S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
4604 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey,D. melanogaster, Endogenous 45 Rabbit
IP 1:50
F 1:100
IF-F 1:400

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry, IF-F=Immunofluorescence (Frozen),

Specificity / Sensitivity

Doublecortin Antibody detects endogenous levels of total doublecortin protein.

Doublecortin Antibody 兔单抗识别内源性的总Doublecortin蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human doublecortin. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体由合成的人类doublecortin蛋白氨基酸序列对应肽段免疫动物而制成。该抗体使用蛋白A和多肽亲和层析纯化而得。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of SK-N-MC cells, using Doublecortin antibody (blue) compared to a nonspecific negative control antibody (red).

流式细胞仪分析SK-N-MC细胞,使用Doublecortin antibody 兔多抗(蓝)和非特异的阴性对照抗体(红)进行标记。

IF-F

IF-F

Confocal immunofluorescent analysis of Doublecortin Antibody (red) and GFAP (GA5) Mouse mAb #3670 (green) staining the subventricular zone of the striatum in reperfused ischemic rat brain. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

共聚焦免疫荧光分析缺血再灌注大鼠脑室下纹状体区域,使用Doublecortin Antibody 兔多抗(红色)和GFAP (GA5) Mouse mAb 鼠单抗#3670(绿色)进行标记。蓝色伪彩=DRAQ ®#4084(荧光DNA染料)。

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse and rat brain, using Doublecortin Antibody.

Western blot分析大鼠和小鼠大脑提取物,使用的抗体是Doublecortin Antibody 兔多抗。

Western Blotting

Western Blotting

Western blot analysis of extracts from fetal rat brain and rat brain, using Doublecortin Antibody (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). 

Background

Mutations in Doublecortin cause Lissencephaly (smooth brain), a neuronal migration disorder characterized by epilepsy and mental retardation (1). Doublecortin is a microtubule associated protein that stabilizes and bundles microtubules. A conserved doublecortin domain mediates the interaction with microtubules, and interestingly most missense mutations cluster in this domain (2). Kinases JNK, CDK5 and PKA phosphorylate doublecortin. JNK phosphorylates Thr321, Thr331 and Ser334 while PKA phosphorylates Ser47 and CDK5 phosphorylates Ser297 (3-5). Phosphorylation of Ser297 lowers the affinity of doublecortin to microtubules. Furthermore, mutations of Ser297 result in migration defects (5).

Doublecortin的突变可以导致无脑回畸形,一种伴有癫痫发作和智力迟滞的神经迁移障碍(1)。Doublecortin是一个微管相关的蛋白,可以稳定和束集微管蛋白。Doublecortin中的一个保守结构域介导其与微管的相互作用,有趣的是,大多数无义突变都发生在这个区域(2)。JNK、CDK5和PKA激酶可以磷酸化Doublecortin。JNK磷酸化Thr321、Thr331和Ser334;PKA磷酸化Ser47;CDK5磷酸化Ser297(3-5)。Ser297的磷酸化降低了Doublecortin与微管的结合。另外,Ser297突变可以导致迁移障碍(5)。

  1. Gleeson, J.G. et al. (1998) Cell 92, 63-72.
  2. Reiner, O. et al. (2004) Cell Cycle 3, 747-51.
  3. Gdalyahu, A. et al. (2004) EMBO J 23, 823-32.
  4. Schaar, B.T. et al. (2004) Neuron 41, 203-13.
  5. Tanaka, T. et al. (2004) Neuron 41, 215-27.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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