Cell Signaling Technology

Product Pathways - Autophagy Signaling

LC3A (D50G8) XP® Rabbit mAb #4599

lc3   lc3-a  

No. Size Price
4599S 100 µl ( 10 western blots ) ¥3,580.00 现货查询 购买询价
4599T 20 µl ( 2 western blots ) ¥1,400.00 现货查询 购买询价
4599 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 14, 16 Rabbit IgG
IHC-P 1:6400
F 1:100
IF-IC 1:400

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Homology

Species predicted to react based on 100% sequence homology: Monkey, Dog,

Specificity / Sensitivity

LC3A (D50G8) XP® Rabbit mAb detects endogenous levels of total LC3A protein. This antibody may also react with LC3B.

LC3A (D50G8) XP® Rabbit mAb 兔单抗能检测内源性的LC3A总蛋白,此抗体也可能与LC3B反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human LC3A.

该单克隆抗体是采用合成的人LC3A氨基末端周围残基相对应的肽段免疫动物制备。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa and NIH/3T3 cells, untreated or chloroquine-treated (50 μM, overnight), using LC3A (D50G8) XP® Rabbit mAb.

Western blot分析HeLa和NIH/3T3细胞的提取物,未处理或氯喹处理(50 μM, 过夜),使用的抗体是LC3A (D50G8) XP® Rabbit mAb 兔单抗。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using LC3A (D50G8) XP® Rabbit mAb (left) orRabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).

免疫组化分析石蜡包埋的人结肠癌,使用LC3A (D50G8) XP® Rabbit mAb 兔单抗(左边)或 Rabbit (DA1E) mAb IgG XP® Isotype Control #3900(右边)。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human glioblastoma multiforme using LC3A (D50G8) XP® Rabbit mAb.

免疫组化分析石蜡包埋的人多形性胶质母细胞瘤,使用的抗体是LC3A (D50G8) XP® Rabbit mAb 兔单抗。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Chloroquine-treated HeLa cells, using LC3A (D50G8) XP® Rabbit mAb (blue) compared to Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red).

流式细胞术分析氯喹处理的HeLa细胞,使用的抗体是LC3A (D50G8) XP® Rabbit mAb 兔单抗(蓝色)Rabbit (DA1E) mAb IgG XP® Isotype Control #3900(红色)作为对照。

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells, untreated (left) or chloroquine-treated (right), using LC3A (D50G8) XP® Rabbit mAb (green). Actin filaments were labeled using DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

共聚焦免疫荧光分析HeLa细胞,未处理(左)或氯喹处理(右),使用的抗体是LC3A (D50G8) XP® Rabbit mAb 兔单抗(绿色)。肌动蛋白丝用DY-554 phalloidin 标记(红色)。蓝色伪彩= DRAQ5® #4084(DNA荧光染料)。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded HeLa cell pellets, control (left) or choloroquine-treated (right), using LC3A (D50G8) XP® Rabbit mAb.

免疫组化分析石蜡包埋的HeLa细胞沉淀,对照(左边)或氯喹处理的(右边),使用的抗体是LC3A (D50G8) XP® Rabbit mAb 兔单抗。

IF-IC

IF-IC

Confocal immunofluorescent analysis of HCT-116 cells, untreated (left) or choroquine-treated (50 uM, overnight; right) using LC3A (D50G8) XP® Rabbit mAb (green) and β-Catenin (L54E2) Mouse mAb (Alexa Fluor® 555 Conjugate) #5612 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegenerative diseases, infection and cancer (3). Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3) (4), and subsequently found to contain similarity to the yeast protein Apg8/Aut7/Cvt5 critical for autophagy (5). Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo post-translational modifications during autophagy (6-9). Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form. During autophagy, LC3-I is converted to LC3-II through lipidation by a ubiquitin-like system involving Atg7 and Atg3 that allows for LC3 to become associated with autophagic vesicles (6-10). The presence of LC3 in autophagosomes and the conversion of LC3 to the lower migrating form LC3-II have been used as indicators of autophagy (11).

自噬是自噬溶酶体对其包裹的细胞质内含物进行降解的一种分解代谢过程(1,2)。自噬过程一般是在营养匮乏的条件下被激活,但是也与一些生理过程有关,如发育、分化、神经变性、感染和癌症(3)。自噬微管相关蛋白的轻链3(LC3)最初被认定为是微管相关蛋白1A和1B的一个亚基(被称为MAP1LC3)(4),随后发现该蛋白所包含的与酵母蛋白类似的Apg8/Aut7/Cvt5对于自噬起着至关重要的作用(5)。三种人类LC3亚型(LC3A、LC3B和LC3C)在自噬过程中翻译后被修饰(6-9)。随着合成后LC3羧基末端迅速裂解,产生了胞浆的LC3-I 型。在自噬过程中,通过一个涉及Atg7和Agt3的类泛素体系,LC3-I被脂质化为LC3-II,从而将LC3与自噬小泡联系起来(6-10)。自噬体中LC3的存在,及其向低迁移形式的LC3-II的转化被作为自噬发生的“指示器”(11)。

  1. Reggiori, F. and Klionsky, D.J. (2002) Eukaryot. Cell 1, 11-21.
  2. Codogno, P. and Meijer, A.J. (2005) Cell Death Differ. 12 Suppl 2, 1509-18.
  3. Levine, B. and Yuan, J. (2005) J. Clin. Invest. 115, 2679-88.
  4. Mann, S.S. and Hammarback, J.A. (1994) J. Biol. Chem. 269, 11492-97.
  5. Lang, T. et al. (1998) EMBO J. 17, 3597-607.
  6. Kabeya, Y. et al. (2000) EMBO J. 19, 5720-28.
  7. He, H. et al. (2003) J. Biol. Chem. 278, 29278-87.
  8. Tanida, I. et al. (2004) J. Biol. Chem. 279, 47704-10.
  9. Wu, J. et al. (2006) Biochem. Biophys. Res. Commun. 339, 437-42.
  10. Ichimura, Y. et al. (2000) Nature 408, 488-92.
  11. Kabeya, Y. et al. (2004) J. Cell Sci. 117, 2805-12.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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