Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Ku70 (D10A7) Rabbit mAb #4588

sc-56129   sc-9033  

No. Size Price
4588S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
4588 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 70 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Hamster, Bovine, Pig,

Specificity / Sensitivity

Ku70 (D10A7) Rabbit mAb recognizes endogenous levels of total Ku70 protein. Ku70 (D10A7) Rabbit mAb能够识别内源性Ku70总蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val294 of mouse Ku70 protein. 该单克隆抗体是由合成的针对小鼠Ku70蛋白缬氨酸(294位)的肽段免疫动物生产的。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Ku70 (D10A7) Rabbit mAb. Western blot方法检测不同细胞提取物,使用的抗体为Ku70 (D10A7) Rabbit mAb。


Ku is a heterodimeric protein composed of two subunits (Ku70 and Ku80) originally identified by researchers as autoantigens associated with several autoimmune diseases including scleroderma, polymyositis, and systemic lupus erythematosus (1). Ku is an abundant, ubiquitously expressed nuclear protein that binds to and stabilizes the ends of DNA at telomeres or double-stranded DNA breaks (2-5). The Ku70/Ku80 heterodimer has ATP-dependent DNA helicase activity and functions as the DNA-binding regulatory component of DNA-dependent protein kinase (DNA-PK) (6-8). The assembly of the DNA-PK complex at DNA ends is required for nonhomologous end-joining (NHEJ), one mechanism involved in double-stranded DNA break repair and V(D)J recombination (8). DNA-PK has been shown to phosphorylate many proteins, including p53, serum response factor, c-Jun, c-Fos, c-Myc, Oct-1, Sp-1, and RNA polymerase II (1,8). The combined activities of Ku70/Ku80 and DNA-PK implicate Ku in many cellular functions, including cell cycle regulation, DNA replication and repair, telomere maintenance, recombination, and transcriptional activation. Ku是由两个亚基(Ku70 and Ku80)组成的异源二聚体蛋白,最初在几种自体免疫疾病包括硬皮病、多发性肌炎和系统性红斑狼疮中作为自体抗原被鉴定出来(1)。Ku是一种高丰度、广泛表达的核蛋白,它能够结合并稳定端粒或双链DNA断裂处的DNA末端(2-5)。Ku70/Ku80异源二聚体具有ATP-依赖的DNA解旋酶活性,并能够作为DNA-依赖蛋白激酶(DNA-PK)的DNA-结合调节成分发挥作用(6-8)。DNA末端DNA-PK复合体的装配需要非同源末端连接(NHEJ),一种涉及双链DNA断裂修复和V(D)J重组的机制(8)。DNA-PK能够磷酸化许多蛋白,包括p53,血清反应因子,c-Jun,c-Fos,c-Myc,Oct-1,Sp-1 和RNA 聚合酶 II (1,8)。Ku70/Ku80和DNA-PK的联合活性暗示了Ku在多种细胞功能中的作用,包括细胞周期调控、DNA复制和修复、端粒维持、重组和转录激活。

  1. Tuteja, R. and Tuteja, N. (2000) Crit. Rev. Biochem. Mol. Biol. 35, 1-33.
  2. Blier, P.R. et al. (1993) J. Biol. Chem. 268, 7594-7601.
  3. Jin, S. and Weaver, D.T. (1997) EMBO J. 16, 6874-6885.
  4. Boulton, S.J. and Jackson, S.P. (1998) EMBO J. 17, 1819-1828.
  5. Gravel, S. et al. (1998) Science 280, 741-744.
  6. Cao, Q.P. et al. (1994) Biochemistry 33, 8548-8557.
  7. Lees-Miller, S.P. et al. (1990) Mol. Cell Biol. 10, 6472-6481.
  8. Collis, S.J. et al. (2005) Oncogene 24, 949-961.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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