Cell Signaling Technology

Product Pathways - Neuroscience

Phospho-PSD95 (Ser295) (A8F8Z) Rabbit mAb #45737

No. Size Price
45737S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
45737 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 95 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-PSD95 (Ser295) (A8F8Z) Rabbit mAb recognizes endogenous levels of PSD95 protein only when phosphorylated at Ser295.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser295 of human PSD95 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse brain, untreated (-) or λ phosphatase-treated (+), using Phospho-PSD95 (Ser295) (A8F8Z) Rabbit mAb (upper) or PSD95 (D74D3) XP® Rabbit mAb #3409 (lower).

Background

Postsynaptic Density protein 95 (PSD95) is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. These family members consist of an amino-terminal variable segment followed by three PDZ domains, a SH3 domain, and an inactive guanylate kinase (GK) domain. PSD95 is a scaffolding protein involved in the assembly and function of the postsynaptic density complex (1-2). PSD95 participates in synaptic targeting of AMPA receptors through an indirect manner involving Stargazin and related transmembrane AMPA receptor regulatory proteins (TARPs) (3). It is implicated in experience-dependent plasticity and plays an indispensable role in learning (4). Mutations in PSD95 are associated with autism (5).

JNK1 phosphorylates PSD95 at Ser295, enhancing synaptic accumulation of PSD95 and potentiating excitatory post-synaptic currents through PSD95's increased ability to recruit AMPA receptors. In addition, synaptic depression requires dephosphorylation of Ser295 (6).

  1. Cao, J. et al. (2005) J. Cell Biol 168, 117-26.
  2. Chetkovich, D.M. et al. (2002) J. Neurosci. 22, 6415-25.
  3. Cai, C. et al. (2006) J. Biol. Chem. 281, 4267-73.
  4. Yao, W.D. et al. (2004) Neuron 41, 625-38.
  5. Cline, H. (2005) Curr. Biol. 15, R203-5.
  6. Kim, M.J. et al. (2007) Neuron 56, 488-502.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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