Cell Signaling Technology

Product Pathways - Protein Translation

TFII-I Antibody #4562

BAP-135   SPIN   TF2   Williams-Beuren syndrome chromosome region 6 protein  

No. Size Price
4562S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
4562 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Monkey, Endogenous 135, 138 Rabbit
IP 1:50
IHC-P 1:400

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

TFII-I Antibody detects endogenous levels of total TFII-I (all isoforms).

TFII-I Antibody能够检测内源性TFII-I (所有亚型)的总蛋白水平。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues of human TFII-I. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, HEK293, U-937, COS, C2C12 and WEHI-231 cells, using TFII-I Antibody.

使用TFII-I Antibody,免疫印迹(Western blot)分析HeLa、HEK293、U-937、COS、C2C12 和WEHI-231细胞系中TFII-I的蛋白水平。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using TFII-I Antibody.

使用TFII-I Antibody,免疫组化分析人源肺癌组织石蜡切片。


TFII-I (also known as SPIN and BAP-135) is a mutifunctional transcription factor that facilitates basal transcriptional machinery assembly at the core promotor region, as well as the assembly of the transcriptional activator complex at upstream regulatory sites (1). Four isoforms of TFII-I (alpha, beta, gamma, and delta) form homo- or heteromeric complexes, which may perform different functions on different promoters (1). In B cells, cross-linking of BCR (B cell receptors) leads to TFII-I phosphorylation at Tyr248 by Btk (2). This phosphorylation disrupts the association of Btk and TFII-I and enhances TFII-I transcriptional activity and nuclear localization (2). In nonlymphoid cells, TFII-I is phosphorylated at Tyr248 by Src dependent kinase or JAK2 (3,4). PKG (cGMP-dependent kinase) interacts with and phosphorylates TFII-I at Ser371 and 743, which also promotes TFII-I transcription activity (5). TFII-I activity is also modulated by HDAC3 (Histone Deacetylase 3) through a physical interaction between the two proteins (6).

TFII-I (也称为SPIN和BAP-135)是一个多功能转录因子,它在中心启动子区域有助于基本的转录机制的装配,以及在上游调控位点转录激活复合物的装配(1)。TFII-I (alpha、beta、gamma和delta)的四个亚型形成同源或异源复合物,这可能在不同的启动子中起着不同的功能(1)。在B细胞中,BCR (B细胞受体)的交联导致由Btk激酶引起TFII-I在Tyr248位点的磷酸化(2)。该磷酸化干扰Btk和TFII-I的关联,并且能提高TFII-I转录活性和细胞核定位(2)。在非淋巴细胞中,TFII-I通过Src依赖激酶或JAK2使Tyr248位点发生磷酸化(3,4)。PKG (cGMP-dependent kinase)与TFII-I相互作用,并且使Ser371和743位点磷酸化,这也促进TFII-I转录活性(5)。HDAC3 (Histone Deacetylase 3)也能通过这两个蛋白的相互作用来调节TFII-I的活性(6)。

  1. Roy, A.L. (2001) Gene 274, 1-13.
  2. Novina, C. D. et al. (1999) Mol. Cell. Biol. 19, 5014-5024.
  3. Kim, D. and Cochran, B.H. (2001) Mol. Cell. Biol. 21, 3387-3397.
  4. Cheriyath, V. et al. (2002) J. Biol. Chem. 277, 22798-22805.
  5. Casteel, D. E. et al. (2002) J. Biol. Chem. 277, 32003-32014.
  6. Wen, Y. et al. (2003) J. Biol. Chem. 278, 1841-1847.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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