Cell Signaling Technology

Product Pathways - Neuroscience

Phospho-MAP2 (Ser136) Antibody #4541

No. Size Price
4541S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
4541 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Rat,Monkey, Endogenous 75, 82, 280 Rabbit
IF-F 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IF-F=Immunofluorescence (Frozen),


Species predicted to react based on 100% sequence homology: Mouse,

Specificity / Sensitivity

Phospho-MAP2 (Ser136) Antibody detects endogenous levels of all isoforms of MAP2 only when phosphorylated at serine 136. This antibody does not cross-react with phosphorylated tau.

Phospho-MAP2 (Ser136) Antibody 兔多抗识别内源性的Ser136磷酸化的MAP2蛋白。此抗体与磷酸化的tau蛋白有交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser136 of human MAP2. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from COS cells, untransfected (lane 1) or MAP2C-transfected (lanes 2,3), using Phospho-MAP2 (Ser136) Antibody (upper) or MAP2 Antibody #4542 (lower). In lane 3, the lysate was treated with lamda phosphatase to demonstrate phospho-specificity of the antibody. (Human MAP2C construct is a generous gift from Dr. B. Shafit-Zagardo, Dept. of Pathology, Albert Einstein College of Medicine, Bronx, NY.)

Western blot分析COS细胞,未转染(列1)或转染MAP2C(列2、3),使用的抗体是Phospho-MAP2 (Ser136) Antibody 兔多抗(上图)或MAP2 Antibody 兔多抗 #4542(下图)。列3中,裂解液经过λ磷酸酶处理,以显示抗体的磷酸化特异性。(人HAP2C载体由 Dept. of Pathology, Albert Einstein College of Medicine, Bronx, NY的Dr. B. Shafit-Zagardo赠送)

Western Blotting

Western Blotting

Western blot analysis of extracts from PC12 cells, untreated or nocodazole-treated, using Phospho-MAP2 (Ser136) Antibody (upper) or MAP2 Antibody #4542 (lower).

Western blot分析PC12细胞提取物,未处理或nocodazole处理,使用的抗体是Phospho-MAP2 (Ser136) Antibody 兔多抗(上图)或MAP2 Antibody 兔多抗 #4542(下图)。



Confocal immunofluorescent analysis of rat brain, untreated (left) or λ-phosphatase treated (right), using Phospho-MAP2 (Ser136) Antibody (green) and β3-Tubulin (TU-20) Mouse mAb #4466 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

共聚焦免疫荧光分析大鼠大脑,未经处理(左图)或经过λ磷酸酶处理(右图),使用的抗体是Phospho-MAP2 (Ser136) Antibody 兔多抗(绿色)和β3-Tubulin (TU-20) Mouse mAb 鼠单抗 #4466(红色)。蓝色伪彩=DRAQ5® #4084(荧光DNA染料)。


Microtubule-associated protein 2 (MAP2) is a neuronal phosphoprotein that regulates the structure and stability of microtubules, neuronal morphogenesis, cytoskeleton dynamics and organelle trafficking in axons and dendrites (1). Multiple MAP2 isoforms are expressed in neurons, including high molecular weight MAP2A and MAP2B (280 and 270 kDa), and low molecular weight MAP2C and MAP2D (70 and 75 kDa). Phosphorylation of MAP2 modulates its association with the cytoskeleton and is developmentally regulated. Serine 136, threonine 1620 and threonine 1623 of MAP2 are phosphorylated by GSK3 and p44/42 MAP kinases (2,3). Phosphorylation at threonine 1620/1623 by GSK3 inhibits MAP2 association with microtubules and microtubule stability (3).

微管相关蛋白2(MAP2)是一种神经元磷酸化蛋白,调节轴突和树突中微管的结构和稳定性,神经元的形态,细胞骨架动力学和细胞器的运输(1)。MAP2的多种亚型在神经元表达,其中包括超高分子量MAP2A和MAP2B(280和270 kDa),低分子量MAP2C和MAP2D(70和75 kDa)。微管相关蛋白的磷酸化调节其与细胞骨架的结合,并受到发育的调控。MAP2的丝氨酸136,苏氨酸1620和苏氨酸1623的磷酸化由GSK3和p44/42 MAP激酶完成(2,3)。GSK3对苏氨酸1620/1623的磷酸化抑制MAP2与微管蛋白结合,削弱微管稳定性(3)。

  1. Sanchez, C. et al. (2000) Prog. Neurobiol. 61, 133-168.
  2. Berling, B. et al. (1994) Eur. J. Cell Biol. 64, 120-130.
  3. Sanchez, C. et al. (2000) Eur. J. Cell Biol. 79, 252-260.

Application References

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