Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Phospho-cdc2 (Tyr15) (10A11) Rabbit mAb #4539

cdc2   cdk1  

No. Size Price
4539L 300 µl ( 30 western blots ) ¥8,792.00 现货查询 购买询价
4539S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
4539T 20 µl ( 2 western blots ) ¥1,300.00 现货查询 购买询价
4539 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 34 Rabbit
IP 1:100
F 1:25
IF-IC 1:25

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Phospho-cdc2 (Tyr15) (10A11) Rabbit mAb detects endogenous levels of cdc2 protein only when phosphorylated at tyrosine 15. Phospho-cdc2 (Tyr15) (10A11) Rabbit mAb 能够检测内源性酪氨酸(15位)磷酸化的cdc2蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr15 of human cdc2. 该单克隆抗体是由合成的人源的针对cdc2蛋白酪氨酸(15位)磷酸化肽段免疫动物而生产的。

Western Blotting

Western Blotting

Western blot analysis of nonphosphorylated GST-cdc2 fusion protein (lane 1), and extracts from SK-N-MC cells treated with hydroxyurea for 20 hours (lane 2), using Phospho-cdc2 (Tyr15) (10A11) Rabbit mAb (upper), or cdc2 Antibody #9112 (lower). Western blot方法检测非磷酸化GST-cdc2融合蛋白(泳道1)和羟基脲处理20小时的SK-N-MC细胞提取物(泳道2),使用的抗体为Phospho-cdc2 (Tyr15) (10A11) Rabbit mAb (上图), 或 cdc2 Antibody #9112 (下图).

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated or hydroxyurea treated for 20 hours, using Phospho-cdc2 (Tyr15) (10A11) Rabbit mAb. western blot方法检测未处理和羟基脲处理20小时的HeLa细胞提取物,使用的抗体为Phospho-cdc2 (Tyr15) (10A11) Rabbit mAb。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, using Phospho-cdc2 (Tyr15) (10A11) Rabbit mAb versus propidium iodide (DNA content). 流式细胞术分析Jurkat细胞,使用的抗体为Phospho-cdc2 (Tyr15) (10A11) Rabbit mAb ,PI染色法检测DNA含量。



Confocal immunofluorescent analysis of asynchronous HeLa cells labeled with Phospho-cdc2 (Tyr15) (10A11) Rabbit mAb (green) and Phospho-Histone H3 (Ser10) (6G3) Mouse mAb #9706 (red). 共聚焦免疫荧光方法分析非同步HeLa细胞,标记的抗体为Phospho-cdc2 (Tyr15) (10A11) Rabbit mAb (绿色) 和 Phospho-Histone H3 (Ser10) (6G3) Mouse mAb #9706 (红色)。


The entry of eukaryotic cells into mitosis is regulated by cdc2 kinase activation, a process controlled at several steps including cyclin binding and phosphorylation of cdc2 at Thr161 (1). However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of cdc2 at Thr14 and Tyr15 (2). Phosphorylation at Thr14 and Tyr15, resulting in inhibition of cdc2, can be carried out by Wee1 and Myt1 protein kinases (3,4). The cdc25 phosphatase may be responsible for removal of phosphates at Thr14 and Tyr15 and subsequent activation of cdc2 (1,5). cdc2蛋白激酶的激活参与调节真核细胞进入有丝分裂,cdc2的激活受多个步骤的调控,包括周期蛋白的结合、cdc2苏氨酸161位的磷酸化(1)。然而,在细胞进入有丝分裂的过程中,激活cdc2的决定性步骤是苏氨酸14位和15位的去磷酸化(2)。Wee1 和 Myt1蛋白激酶通过磷酸化cdc2苏氨酸14位和15位,抑制cdc2活性(3,4)。而cdc25磷酸酯酶可能起到使cdc2苏氨酸14位和15位去磷酸化从而激活cdc2的作用(1,5)。

  1. Atherton-Fessler, S. et al. (1994) Mol Biol Cell 5, 989-1001.
  2. Norbury, C. et al. (1991) EMBO J 10, 3321-9.
  3. McGowan, C.H. and Russell, P. (1993) EMBO J 12, 75-85.
  4. Wells, N.J. et al. (1999) J Cell Sci 112 ( Pt 19), 3361-71.
  5. Hunter, T. (1995) Cell 80, 225-36.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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