Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-TAK1 (Thr187) Antibody #4536

MAP3K7   Mitogen-activated protein kinase kinase kinase 7   Tranforming growth factor-activated kinase 1  

No. Size Price
4536S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
4536 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 82 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat, Chicken, Xenopus, Zebrafish, Bovine,

Specificity / Sensitivity

Phospho-TAK1 (Thr187) Antibody detects endogenous levels of TAK1 only when phosphorylated at threonine 187.

Phospho-TAK1 (Thr187) Antibody兔多抗能够检测内源性Thr187位点磷酸化的TAK1蛋白水平。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr187 of human TAK1. Antibodies are purified by protein A and affinity chromatography.

该多克隆抗体是采用合成的与人源TAK1蛋白Thr187位点周围残基相一致的磷酸化肽段免疫动物而获得。该抗体经蛋白A和肽亲和层析纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293IL-1R cells, untreated or treated with IL-1beta and Calyculin A for the indicated times, using Phospho-TAK1 (Thr187) Antibody.

Background

TAK1 is a mitogen-activated protein kinase kinase kinase that can be activated by TGF-β, bone morphogenetic protein and other cytokines including IL-1 (1,2). In vivo activation of TAK1 requires association with TAK1 binding protein 1 (TAB1), which triggers phosphorylation of TAK1 (3,4). Another adaptor protein, TAB2, links TAK1 with TRAF6 and mediates TAK1 activation upon IL-1 stimulation (5). Once activated, TAK1 phosphorylates MAPK kinases MKK4 and MKK3/6, which activate p38 MAPK and JNK, respectively. In addition, TAK1 activates the NF-κB pathway by interacting with TRAF6 and phosphorylating the NF-κB inducing kinase (NIK) (2).

TAK1是MAPKKK家族中的成员,由TGF-β、骨形态生成蛋白和包括IL-1在内的细胞因子所激活(1,2)。在体内,TAK1的活化需要它与TAK1结合蛋白1 (TAB1)的结合,这种结合能够使TAK1蛋白磷酸化(3,4)。另一个接头蛋白TAB2能够将TAK1与TRAF6连接起来,调节IL-1刺激引起的TAK1的活化(5)。一经激活,TAK1能够磷酸化MAPK激酶MKK4和MKK3/6,MKK4和MKK3/6再分别激活JNK和p38 MAPK。另外,TAK1能够激活NF-κB通路,这是通过与TRAF6的相互作用及对NF-κB诱导激酶(NIK)的磷酸化实现的 (2)。

TAK1 activation requires multiple phosphorylations in its activation loop. Mutation of Thr187 and Thr184, residues located in the activation loop of TAK1, impairs phosphorylation of both TAK1 and TAB1 and reduces the kinase activity of TAK1, suggesting that autophosphorylation of these residues is necessary for TAK1 activation (4).

TAK1的活化需要活化环内的多位点磷酸化。定位于TAK1活化环内的Thr187和Thr184位点的突变,会影响TAK1、TAB1的磷酸化,降低TAK1的激酶活性,暗示了这些位点的自磷酸化是TAK1活化所必需的(4)。

  1. Yamaguchi, K. et al. (1995) Science 270, 2008-2011.
  2. Ninomiya-Tsuji, J. et al. (1999) Nature 398, 252-256.
  3. Shibuya, H. et al. (1996) Science 272, 1179-1182.
  4. Sakurai, H. et al. (2000) FEBS Lett. 474, 141-145.
  5. Takaesu, G. et al. (2000) Mol. Cell 4, 649-658.
  6. Sakurai, H. et al. (2000) FEBS 474, 141-145.

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For Research Use Only. Not For Use In Diagnostic Procedures.

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