Product Pathways - Chromatin Regulation / Epigenetics
RBAP46 Antibody #4522
|4522S||100 µl ( 10 western blots )||￥3,250.00 现货查询||购买询价|
|4522||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting,
Species predicted to react based on 100% sequence homology: Bovine,
Specificity / Sensitivity
RBAP46 Antibody detects endogenous levels of total RBAP46 protein. The antibody does not cross-react with RBAP48 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of the human RBAP46 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Retinoblastoma-associated proteins 46 and 48 (RBAP46 and RBAP48; also known as RBBP7 and RBBP4) were first characterized in human cells as proteins that bind to the retinoblastoma (Rb) tumor suppressor protein (1). Since then, these proteins have been shown to be components of many protein complexes involved in chromatin regulation, including the chromatin assembly factor 1 (CAF-1) complex and type B histone acetyltransferase complex HAT1, both of which function in chromatin assembly during DNA replication (2,3). RBAP46 and RBAP48 are also found in the nucleosome remodeling factor complex NURF, the nucleosome remodeling and histone de-acetylation complex NuRD, and the Sin3/HDAC histone de-acetylation complex (4-7). More recently, RBAB46 and RBAP48 were identified as components of the polycomb repressor complex PRC2, which also contains EED and Ezh2 (8). RBAP46 and RBAP48 bind to the histone fold region of histone H4 and are believed to target these chromatin remodeling, histone acetylation, and histone de-acetylation complexes to their histone substrates (3).
Retinoblastoma-associated proteins 46和48 (RBAP46和RBAP48;又称为RBBP7和RBBP4)在人源细胞中首先以结合到retinoblastoma (Rb)肿瘤抑制蛋白为特征(1)。从那时以来，这些蛋白质已经显示是许多蛋白质复合物中的成分，这些复合物涉及染色质调节，包括chromatin assembly factor 1 (CAF-1)复合物和type B组蛋白乙酰转移酶复合物HAT1，在DNA复制期间这两个复合物的功能是染色质的装配(2,3)。RBAP46和RBAP48蛋白也被发现在核小体重构因子复合物NURF、核小体重塑和组蛋白去乙酰化复合物NuRD和Sin3/HDAC组蛋白去乙酰化复合物(4-7)。最近，RBAB46和RBAP48被鉴定作为polycomb repressor complex PRC2的成分，该复合物也包含EED和Ezh2 (8)。RBAP46和RBAP48结合到组蛋白histone H4折叠区域，以及认为有助于这些染色质重塑、组蛋白乙酰化和组蛋白去乙酰化复合物作用于它们的组蛋白底物上(3)。
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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