Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-SEK1/MKK4 (Ser257) (C36C11) Rabbit mAb #4514

mkk346   mkk47  

No. Size Price
4514S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
4514T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
4514 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 44 Rabbit
F 1:400

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, F=Flow Cytometry,

Specificity / Sensitivity

Phospho-SEK1/MKK4 (Ser257) (C36C11) Rabbit mAb detects endogenous levels of SEK1/MKK4 phosphorylated at Ser257 .

Phospho-SEK1/MKK4 (Ser257) (C36C11) Rabbit mAb兔单抗能检测内源性Ser257位点磷酸化的SEK1/MKK4蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser257/Thr261 of human SEK1/MKK4.

该单克隆抗体通过使用与人源SEK1/MKK4蛋白Ser257/Thr261位点周围残基相一致的合成磷酸化肽段免疫动物而获得。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines, untreated or treated with sorbitol or UV, as indicated, using Phospho-SEK1/MKK4 (Ser257) (C36C11) Rabbit mAb.western blot方法检测细胞提取物:未处理、根据说明用山梨醇处理的和紫外照射的各种细胞系。使用的抗体是Phospho-SEK1/MKK4 (Ser257) (C36C11) Rabbit mAb。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HT-29 cells, untreated (blue) or UV-treated (green), using Phospho-SEK1/MKK4 (Ser257) (C36C11) Rabbit mAb compared to a nonspecific negative control antibody (red).Flow Cytometry方法检测细胞提取物:未经处理(蓝色)的和紫外照射的(绿色)HT-29细胞,使用的抗体是Phospho-SEK1/MKK4 (Ser257) (C36C11) Rabbit mAb,红色表示非特异性阴性对照抗体。

Background

SAPK/Erk kinase (SEK1), also known as MKK4 or Jun kinase kinase (JNKK), activates the MAP kinase homologues SAPK and JNK in response to various cellular stresses and inflamatory cytokines (1-3). Activation of SEK1 occurs through MEKK phosphorylation of serine and threonine residues at positions 257 and 261, respectively. Like MEK, SEK is a dual-specificity protein kinase that phosphorylates SAPK/JNK at a conserved T*PY* site in its activation loop (4). Phosphorylation by Akt at Ser80 inhibits SEK1 and suppresses stress-activated signal transduction (5).

SAPK/Erk 激酶(SEK1),也被称为MKK4或Jun激酶的激酶 (JNKK),能激活MAPK同系物SAPK、 JNK,以应答各种细胞应激反应和炎性细胞因子(1-3)。SEK1的活化,是通过MEKK对其Ser257位点和Thr261位点的磷酸化实现的。类似于MEK,SEK也是一种双特异性蛋白激酶,能将SAPK/JNK活化环内的保守位点T*PY* 磷酸化(4)。Akt对SEK1的Ser80位点磷酸化作用,能够抑制SEK1以及应激活化的信号转导(5)。

  1. Davis, R.J. (1994) Trends Biochem. Sci. 19, 470-473.
  2. Sanchez, I. et al. (1994) Nature 372, 794-798.
  3. Yan, M. et al. (1994) Nature 372, 798-800.
  4. Kyriakis, J.M. et al. (1994) Nature 369, 156-160.
  5. Park, H. et al. (2002) J. Biol. Chem. 277, 2573-2578.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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