Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

Phospho-PLCγ1 (Ser1248) Antibody #4510


No. Size Price
4510S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
4510 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 155 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-PLCγ1 (Ser1248) Antibody detects PLCγ1 only when phosphorylated at Ser1248.

Phospho-PLCγ1 (Ser1248)抗体识别内源性的Ser1248磷酸化的PLCγ1蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser1248 of human PLCγ1. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, untreated or PDGF-treated, using Phospho-PLCγ1 (Ser1248) Antibody (upper) or PLCγ1 Antibody #2822 (lower).

对未处理或用PDGF刺激的NIH/3T3细胞抽提液物,使用Phospho-PLCγ1 (Ser1248)抗体(上图)或PLCγ1抗体#2822(下图)进行Western blot分析。

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat or COS cells, untreated or TPA/UV treated, using Phospho-PLCγ1 (Ser1248) Antibody.

对Jurkat或COS细胞,未处理或TPA/UV处理,使用Phospho-PLCγ1 (Ser1248)抗体进行Western blot分析。


Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli such as hormones, growth factors and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate two secondary messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) (1). At least four families of PLCs have been identified: PLCβ, PLCγ, PLCδ and PLCε. The PLCβ subfamily includes four members, PLCβ1-4. All four members of the subfamily are activated by α- or β-γ-subunits of the heterotrimeric G-proteins (2,3). 
 Phosphorylation is one of the key mechanisms that regulates the activity of PLC. Phosphorylation of Ser1105 by PKA or PKC inhibits PLCβ3 activity (4,5). Ser537 of PLCβ3 is phosphorylated by CaMKII, and this phosphorylation may contribute to the basal activity of PLCβ3. PLCγ is activated by both receptor and nonreceptor tyrosine kinases (6). 
 PLCγ forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCγ at Tyr771, 783 and 1245 (7). Phosphorylation by Syk at Tyr783 activates the enzymatic activity of PLCγ1 (8).


Phosphorylation of PLCγ1 ar Y783 by EGFR causes a conformational change of PLCγ1 that allows the interaction of its SH3 domain with Akt proline-rich motifs. This interaction results in Akt phosphorylation of PLCγ1 at S1248 by Akt (9).


  1. Singer, W.D. et al. (1997) Annu Rev Biochem 66, 475-509.
  2. Smrcka, A.V. et al. (1991) Science 251, 804-7.
  3. Taylor, S.J. et al. (1991) Nature 350, 516-8.
  4. Yue, C. et al. (1998) J Biol Chem 273, 18023-7.
  5. Yue, C. et al. (2000) J Biol Chem 275, 30220-5.
  6. Margolis, B. et al. (1989) Cell 57, 1101-7.
  7. Kim, H.K. et al. (1991) Cell 65, 435-41.
  8. Wang, Z. et al. (1998) Mol Cell Biol 18, 590-7.
  9. Wang, Y. et al. (2006) Mol. Biol. Cell 17, 2267-2277.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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