Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-TAK1 (Thr184/187) (90C7) Rabbit mAb #4508

No. Size Price
4508L 300 µl ( 30 western blots ) ¥8,792.00 现货查询 购买询价
4508S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
4508 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 82 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Mouse, Rat, Chicken, Xenopus, Zebrafish, Bovine,

Specificity / Sensitivity

Phospho-TAK1 (Thr184/187) (90C7) Rabbit mAb detects endogenous levels of TAK1 only when phosphorylated at threonine 184 and threonine 187. In some cell lysates, the antibody may cross-react with a 40 kDa band of unknown origin.

Phospho-TAK1 (Thr184/187) (90C7) Rabbit mAb兔单抗能够检测内源性Thr184/187位点磷酸化的TAK1蛋白水平。在一些细胞裂解物中,该抗体可能与未知来源的40 kDa蛋白发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a phosphopeptide corresponding to residues surrounding Thr184 and Thr187 of human TAK1.


Western Blotting

Western Blotting

Western blot analysis of extracts from 293 IL-1R cells, untreated or treated for 10 minutes with IL-1, using Phospho-TAK1 (Thr184/187) (90C7) Rabbit mAb (upper) or TAK1 Antibody #4505 (lower).western blot方法检测细胞提取物: 未经处理和IL-1 10 min处理的293IL-1R细胞。使用的抗体是Phospho-TAK1 (Thr184/187) (90C7) Rabbit mAb (上图)、TAK1 Antibody #4505 (下图)。


TAK1 is a mitogen-activated protein kinase kinase kinase that can be activated by TGF-β, bone morphogenetic protein and other cytokines including IL-1 (1,2). In vivo activation of TAK1 requires association with TAK1 binding protein 1 (TAB1), which triggers phosphorylation of TAK1 (3,4). Another adaptor protein, TAB2, links TAK1 with TRAF6 and mediates TAK1 activation upon IL-1 stimulation (5). Once activated, TAK1 phosphorylates MAPK kinases MKK4 and MKK3/6, which activate p38 MAPK and JNK, respectively. In addition, TAK1 activates the NF-κB pathway by interacting with TRAF6 and phosphorylating the NF-κB inducing kinase (NIK) (2).

TAK1是MAPKKK家族中的成员,由TGF-β、骨形态生成蛋白和包括IL-1在内的细胞因子所激活(1,2)。在体内,TAK1的活化需要它与TAK1结合蛋白1 (TAB1)的结合,这种结合能够使TAK1蛋白磷酸化(3,4)。另一个接头蛋白TAB2能够将TAK1与TRAF6连接起来,调节IL-1刺激引起的TAK1的活化(5)。一经激活,TAK1能够磷酸化MAPK激酶MKK4和MKK3/6,MKK4和MKK3/6再分别激活JNK和p38 MAPK。另外,TAK1能够激活NF-κB通路,这是通过与TRAF6的相互作用及对NF-κB诱导激酶(NIK)的磷酸化实现的 (2)。

  1. Yamaguchi, K. et al. (1995) Science 270, 2008-2011.
  2. Ninomiya-Tsuji, J. et al. (1999) Nature 398, 252-256.
  3. Shibuya, H. et al. (1996) Science 272, 1179-1182.
  4. Sakurai, H. et al. (2000) FEBS Lett. 474, 141-145.
  5. Takaesu, G. et al. (2000) Mol. Cell 4, 649-658.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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