Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-Tpl2 (Ser400) Antibody #4491


No. Size Price
4491S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
4491 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Rat, Transfected Only 60, 62 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Human, Mouse,

Specificity / Sensitivity

Phospho-Tpl2 (Ser400) Antibody detects transfected Tpl2 only when phosphorylated at Ser400. This antibody does not cross-react with other phosphorylated MAP kinase kinase kinases.

Phospho-Tpl2 (Ser400)兔多抗能检测转染水平的丝氨酸(400位点)磷酸化的Tpl2蛋白。该抗体不与其他磷酸化的MAP激酶发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a mixture synthetic phosphorylated peptides corresponding to residues surrounding Ser400 of human, mouse and rat Tpl2. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from COS cells, untransfected or transfected with rat Tpl2 , using Phospho-Tpl2 (Ser400) Antibody (upper) and Tpl2 Antibody #4492 (lower). In lane 3, Tpl2 transfected COS cell lysate was treated with λ phosphatase to demonstrate the phospho-specificity of Phospho-Tpl2 (Ser400) Antibody.Western blot方法检测细胞提取物:未处理和转染大鼠Tpl2蛋白的COS细胞。使用的抗体是Phospho-Tpl2 (Ser400) Antibody (上图) 和Tpl2 Antibody #4492 (下图).


Tpl2 (tumor progression locus 2), also known as COT (cancer osaka thyroid), is a serine/threonine kinase expressed primarily in hematopoietic tissues, lung and liver (1). Over-expression of Tpl2 potentiates MAP kinase pathways through MEK1 and SEK1, as well as through MKK6 and MEK5 (2,3). Tpl2 is also engaged in NF-κB activation through NF-κB inducing kinase (NIK), or by inducing phosphorylation and degradation of the NF-κB precusor, p105 NF-κB1 (4,5). Ser400 of Tpl2 is phosphorylated in an Akt-dependent manner. This phosphorylation is required for Tpl2-induced NF-κB-dependent transcription (6). Tpl2 also activates caspase-3 by promoting the assembly of a protein complex of Apaf1 (apoptotic protease-activating factor 1), caspase-9, Tpl2, adaptor protein Tvl1 and procaspase-3 (7).

Tpl2(肿瘤发展位点2)也被称为COT(大阪甲状腺癌)是一种丝氨酸/苏氨酸激酶,主要表达于造血组织、肺和肝脏中(1)。Tpl2的过度表达会通过MEK1和SEK1以及MKK6 和MEK5增强MAP激酶途径(2,3)。Tpl2也能通过NF-κB诱导激酶(NIK)或通过诱导NF-κB前体p105 NF-κB1的磷酸化和降解,参与NF-κB的活化(4,5)。Tpl2蛋白丝氨酸(400位点)被一个依赖Akt的方式磷酸化,该磷酸化是Tpl2诱导的NF-κB依赖型转录所必需的(6)。Tpl2蛋白也能通过促进一种蛋白复合体Apaf1 (凋亡蛋白酶活化因子 1)、caspase-9、 Tpl2、转接蛋白 Tvl1 和酶原-3的聚集而活化caspase-3(7)。

  1. Patriotis, C. et al. (1993) Proc Natl Acad Sci U S A 90, 2251-5.
  2. Salmeron, A. et al. (1996) EMBO J 15, 817-26.
  3. Chiariello, M. et al. (2000) Mol Cell Biol 20, 1747-58.
  4. Lin, X. et al. (1999) Immunity 10, 271-80.
  5. Belich, M.P. et al. (1999) Nature 397, 363-8.
  6. Kane, L.P. et al. (2002) Mol Cell Biol 22, 5962-74.
  7. Patriotis, C. et al. (2001) J Cell Physiol 187, 176-87.

Application References

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