Cell Signaling Technology

Product Pathways - Neuroscience

β3-Tubulin (TU-20) Mouse mAb #4466

beta-III-tubulin   sc-5274   TU20   TUJ   TUJ-1   βtubulin  

No. Size Price
4466S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
4466 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 55 Mouse IgG1
IHC-P 1:100
IF-F 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IF-F=Immunofluorescence (Frozen),

Specificity / Sensitivity

β3-Tubulin (TU-20) Mouse mAb detects endogenous levels of total β3-tubulin protein and does not cross-react with tubulin isoforms expressed in non-neuronal cells. This clone is similar to TUJ1.

β3-Tubulin(TU-20)Mouse mAb 鼠单抗识别内源性的总β3-微管蛋白,并且不与非神经元中表达的tubulin异构体发生交互作用。此克隆类似TUJ1。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human β3-tubulin protein.


Western Blotting

Western Blotting

Western blot analysis of extracts from fetal rat and neonatal mouse brain using β3-Tubulin (TU-20) Mouse mAb.

Western blot分析胎大鼠和新生小鼠大脑提取物,使用的抗体是β3-Tubulin (TU-20) Mouse mAb 鼠单抗。



Confocal immunofluorescent image of rat cerebellum labeled with β3-Tubulin (TU-20) Mouse mAb (green) and Neurofilament-L (C28E10) Rabbit mAb #2837 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

共聚焦免疫荧光分析大鼠小脑,使用的抗体是β3-Tubulin (TU-20)Mouse mAb 鼠单抗(绿)和Neurofilament-L (C28E10)Rabbit mAb 兔单抗#2837(红)。蓝色伪彩=DRAQ5®#4084(荧光DNA染料)。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded rat brain using beta3-Tubulin (TU-20) Mouse mAb.

免疫组化分析石蜡包埋的大鼠大脑切片,使用的抗体是β3-Tubulin (TU-20)Mouse mAb 鼠单抗。


The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).


  1. Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47.
  2. Jiang, Y.Q. and Oblinger, M.M. (1992) J Cell Sci 103 ( Pt 3), 643-51.
  3. Panda, D. et al. (1994) Proc Natl Acad Sci U S A 91, 11358-62.
  4. Tischfield, M.A. et al. (2010) Cell 140, 74-87.
  5. Katsetos, C.D. et al. (2003) J Child Neurol 18, 851-66; discussion 867.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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