Cell Signaling Technology

Product Pathways - Neuroscience

β3-Tubulin (TU-20) Mouse mAb #4466

beta-III-tubulin   sc-5274   TU20   TUJ   TUJ-1   βtubulin  

No. Size Price
4466S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
4466 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 55 Mouse IgG1
IHC-P 1:100
IF-F 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IF-F=Immunofluorescence (Frozen),

Specificity / Sensitivity

β3-Tubulin (TU-20) Mouse mAb detects endogenous levels of total β3-tubulin protein and does not cross-react with tubulin isoforms expressed in non-neuronal cells. This clone is similar to TUJ1.

β3-Tubulin(TU-20)Mouse mAb 鼠单抗识别内源性的总β3-微管蛋白,并且不与非神经元中表达的tubulin异构体发生交互作用。此克隆类似TUJ1。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human β3-tubulin protein.

该单克隆抗体由合成的β3-微管蛋白羧基末端附近肽段免疫动物而制成。

Western Blotting

Western Blotting

Western blot analysis of extracts from fetal rat and neonatal mouse brain using β3-Tubulin (TU-20) Mouse mAb.

Western blot分析胎大鼠和新生小鼠大脑提取物,使用的抗体是β3-Tubulin (TU-20) Mouse mAb 鼠单抗。

IF-F

IF-F

Confocal immunofluorescent image of rat cerebellum labeled with β3-Tubulin (TU-20) Mouse mAb (green) and Neurofilament-L (C28E10) Rabbit mAb #2837 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

共聚焦免疫荧光分析大鼠小脑,使用的抗体是β3-Tubulin (TU-20)Mouse mAb 鼠单抗(绿)和Neurofilament-L (C28E10)Rabbit mAb 兔单抗#2837(红)。蓝色伪彩=DRAQ5®#4084(荧光DNA染料)。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded rat brain using beta3-Tubulin (TU-20) Mouse mAb.

免疫组化分析石蜡包埋的大鼠大脑切片,使用的抗体是β3-Tubulin (TU-20)Mouse mAb 鼠单抗。

Background

The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).

细胞骨架由三种胞内纤维构成:微管、微丝(肌动蛋白丝)和中间纤维。球状微管蛋白亚基形成聚集,与α/β微管蛋白异二聚体共同构成所有真核细胞的微管蛋白亚基。γ-微管蛋白是核微管蛋白亚基的聚合形成微管聚合物所必需的。许多细胞运动由微管蛋白活动介导,包括纤毛和鞭毛拍打,细胞质的运输膜泡,染色体在减数分裂/有丝分裂中的排列,以及神经细胞轴突迁移。这些运动是微管聚合和解聚的竞争性活动的结果,或者是微管运动蛋白活动的结果(1)。

  1. Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47.
  2. Jiang, Y.Q. and Oblinger, M.M. (1992) J Cell Sci 103 ( Pt 3), 643-51.
  3. Panda, D. et al. (1994) Proc Natl Acad Sci U S A 91, 11358-62.
  4. Tischfield, M.A. et al. (2010) Cell 140, 74-87.
  5. Katsetos, C.D. et al. (2003) J Child Neurol 18, 851-66; discussion 867.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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