Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

Phospho-Stat2 (Tyr690) Antibody #4441

No. Size Price
4441S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
4441T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
4441 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 113 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Homology

Species predicted to react based on 100% sequence homology: Rat, Bovine,

Specificity / Sensitivity

Phospho-Stat2 Antibody detects endogenous levels of human Stat2 only when phosphorylated at Tyr690. The antibody does not significantly cross-react with corresponding phospho-tyrosine sites on other Stat proteins.

Phospho-Stat2 Antibody只能检测内源的在Tyr690位点磷酸化的Stat2蛋白。此抗体不与对应的酪氨酸磷酸化的Stat家族的其它蛋白交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr690 of human Stat2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

此多克隆抗体是通过合成人源对应的Stat2 Tyr690位点周围的磷肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析法纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated or IFN-α-treated (100 ng/ml, 15 minutes), using Stat2 Antibody (upper) or Phospho-Stat2 (Tyr690) Antibody (lower).Western免疫印迹。用 Stat2 Antibody (上图) or Phospho-Stat2 (Tyr690) Antibody (下图)研究未经处理的和经IFN-alpha(100 ng/ml,15 min)处理的 HeLa 细胞的细胞提取液。

Background

Stat2 (113-kDa), originally purified from the nuclei of alpha-interferon-treated cells, is critical to the transcriptional responses induced by type I interferons, IFN-alpha/beta (1,2). Knockout mice with a targeted disruption of Stat2 have higher susceptibility to viral infection and altered responses to type I interferons (3). Stat2 is rapidly activated by phosphorylation at Tyr690 in response to stimulation by IFN-alpha/beta via associations with receptor-bound Jak kinases (4). Unlike other Stat proteins, Stat2 does not form homodimers. Instead, activated Stat2 forms a heterodimer with Stat1 and translocates to the nucleus. There, it associates with the DNA-binding protein p48 and forms the transcriptional activator complex, interferon-stimulated gene factor 3 (ISGF3), promoting transcription from the ISRE (5).

Stat2 (113kDa),最初是从用alpha干扰素处理的细胞核中提纯,它对类型I干扰素IFN-alpha/beta的诱导的转录响应是非常重要的(1,2)。 针对Stat2基因敲除的小鼠对病毒的感染呈现出易感性对类型I干扰素的反应发生改变(3)。Stat2的激活是在IFN-alpha/beta的诱导下通过受体结合到Jak激酶上而在 Tyr690位点快速被磷酸化(4)。与其它的Stat蛋白不同, Stat2 不形成同源二聚体,激活的Stat2与Stat1形成异源二聚体并定位到细胞核内。在细胞核内它与DNA结合蛋白p48结合并形成转录激活复合体——干扰素刺激因子3(ISGF3),介导从ISRE开始的转录(5)。

  1. Fu, X.Y. et al. (1992) Proc Natl Acad Sci U S A 89, 7840-3.
  2. Ihle, J.N. (2001) Curr Opin Cell Biol 13, 211-7.
  3. Park, C. et al. (2000) Immunity 13, 795-804.
  4. Improta, T. et al. (1994) Proc Natl Acad Sci U S A 91, 4776-80.
  5. Horvath, C.M. et al. (1996) Mol Cell Biol 16, 6957-64.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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