Cell Signaling Technology

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Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate) #4413

secondary  

Applications Species-Reactivity Sensitivity MW (kDa) Isotype
HCA Goat
IF-IC

Species cross-reactivity is determined by western blot.

Applications Key: HCA=High Content Analysis, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

F(ab')2 fragments are prepared from goat antibodies that have been adsorbed against pooled human serum, mouse serum, plasmacytoma/hybridoma proteins and purified human paraproteins.

F(ab’)2片段是采用羊抗体制备的,已经吸附了人血清、鼠血清、浆细胞瘤/杂交瘤蛋白和纯化的人类病变蛋白。

Description

Anti-Rabbit IgG (H+L) F(ab')2 Fragment was conjugated to Alexa Fluor® 555 fluorescent dye under optimal conditions and formulated at 2 mg/ml. This F(ab')2 fragment results in less non-specific binding to cells through Fc receptors.

Anti- Rabbit IgG (H+L) F(ab’)2 Fragment在最佳条件下结合了 Alexa Fluor ® 555荧光染料,浓度为2mg/ml. F(ab’)2片段可以减少通过Fc受体的非特异性结合。

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells labeled with MEK1/2 (47E6) Rabbit mAb #9126 detected with Anti-Rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate) (red, left) compared to an isotype control (right). Actin filaments have been labeled with fluorescein phalloidin (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).激光共聚焦免疫荧光分析HeLa细胞,所用抗体为MEK1/2 (47E6) Rabbit mAb #9126检测抗体为anti- Rabbit IgG (H+L), F(ab’)2 Fragment (Alexa Fluor ® 555 Conjugate) (红色,上图),与同型对照(下图)作比较。肌动蛋白丝采用fluorescein phalloidin (绿色),蓝色伪彩为荧光DNA染料,信息为DRAQ5 ® #4084。

IF-IC

IF-IC

Confocal immunofluorescent analysis of mouse cerebellum using α-Synuclein Antibody (IF Preferred) #2628 detected with Anti-Rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate) (red) and Neurofilament-L (DA2) Mouse mAb #2835 detected with Anti-Mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4408 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).激光共聚焦免疫荧光分析鼠小脑细胞,所用抗体为α-Synuclein Antibody (IF Preferred) #2628检测抗体为anti- Rabbit IgG (H+L), F(ab’)2 Fragment (Alexa Fluor ® 555 Conjugate) (红色)和Neurofilament-L (DA2) Mouse mAb #2835,检测抗体为Anti-Mouse IgG (H+L), F(ab’)2 Fragment (Alexa Fluor ® 488 Conjugate) #4408 (绿色) 。蓝色伪彩为荧光DNA染料,信息为DRAQ5 ® #4084。

High Content Screening

High Content Screening

High content analysis of A549 cells exposed to varying concentrations of LY294002 #9901 for 3 hrs, followed by 100 ng/mL EGF for 20 minutes. With increasing concentrations of LY294002, a significant decrease (~5 fold) in phospho-S6 Ribosomal Protein (Ser235/236) signal as compared to the uninhibited control was observed. When using phospho-S6 as a measurement, the IC50 of this compound was 3.06 μM. Data were generated on the Acumen HCS platform using Anti-Rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate).图 高通量分析A549细胞,该细胞暴露于不同浓度的LY294002 #9901 3个小时。然后用100ng/mlde EGF 处理20分钟。与未抑制组相比较,随着LY294002 #9901浓度的增加phospho-S6 Ribosomal Protein (Ser235/236)信号显著降低(约5倍)。当phospho-S6 Ribosomal Protein 检测时,IC50为3.06mM。数据时以Acumen ® HCS为平台生成的,所用抗体为Anti-Rabbit IgG (H+L), F(ab’)2 Fragment (Alexa Fluor ® 555 Conjugate)。

Applications

This product has been optimized for use as a secondary antibody in immunofluorescent applications.

本产品已经过优化,可以作为二抗应用于免疫荧光中。

Directions for Use

The optimal dilution of the anti-species antibody should be determined for each application. However, a final dilution of 1:1000 should yield acceptable results for most immunofluorescentapplications.

抗体的最佳稀释度取决于每一个应用。然而,1:1000的终稀释度在免疫荧光分析中可以得到好的结果。

Background

Fluorescent anti-species IgG conjugates are ideal for flow cytometry and immunofluorescence. Cell Signaling Technology’s strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.

荧光抗IgG结合物适用于流式细胞术和免疫荧光。CST公司的严格的质控程序保证了每一个结合物具有最佳的特异性和荧光强度。

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

Acumen is a registered trademarks of TTP Labtech.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

The Alexa Fluor dye antibody conjugates in this product are sold under license from Life Technologies Corporation, for research use only for immunocytometry, immunohistochemistry, high content screening (HCS) analysis, or flow cytometry applications.

The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer (whether the buyer is an academic or for-profit entity), for Immunocytochemistry, high content screening (HCS) analysis, or flow cytometry applications. The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) resale, whether or not such product or its components are resold for use in research; or for any other commercial purpose. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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