Cell Signaling Technology

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Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412

secondary  

Applications Species-Reactivity Sensitivity MW (kDa) Isotype
HCA Goat
F
IF-IC

Species cross-reactivity is determined by western blot.

Applications Key: HCA=High Content Analysis, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

F(ab')2 fragments are prepared from goat antibodies that have been adsorbed against pooled human serum, mouse serum, plasmacytoma/hybridoma proteins and purified human paraproteins.

F(ab’)2片段是采用羊抗体制备的,已经吸附了人血清、鼠血清、浆细胞瘤/杂交瘤蛋白和纯化的人类病变蛋白。

Description

Anti-Rabbit IgG (H+L) F(ab')2 Fragment was conjugated to Alexa Fluor® 488 fluorescent dye under optimal conditions and formulated at 2 mg/ml. This F(ab')2 fragment results in less non-specific binding to cells through Fc receptors.

Anti- Rabbit IgG (H+L) F(ab’)2 Fragment在最佳条件下结合了 Alexa Fluor ® 488荧光染料,浓度为2mg/ml. F(ab’)2片段可以减少通过Fc受体的非特异性结合。

IF-IC

IF-IC

Confocal immunofluorescent analysis of P19 cells using LIN28A (A177) Antibody #3978 detected with Anti-Rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).激光共聚焦免疫荧光分析P19细胞,所用抗体为LIN28A (A177) Antibody #3978检测抗体为anti- Rabbit IgG (H+L), F(ab’)2 Fragment (Alexa Fluor ® 488 Conjugate) (绿色)。肌动蛋白丝采用DY-554 phalloidin (红色),蓝色伪彩为荧光DNA染料,信息为DRAQ5 ® #4084。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002 #9901, Wortmannin #9951 and U0126 #9903 (blue), using Phospho-Akt (Ser473) (D9E) Rabbit mAb #4060 detected with Anti-Rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) and compared to a nonspecific negative control antibody (red).流式细胞术分析Jurkat细胞,绿色是未处理组,蓝色为采用LY294002 #9901, Wortmannin #9951 和 U0126 #9903 (blue)处理组。所用抗体为Phospho-Akt (Ser473) (D9E) Rabbit mAb #4060,检测抗体为Anti-Rabbit IgG (H+L), F(ab’)2Fragment (Alexa Fluor ® 488 Conjugate) (绿色) ,与非特异性阴性对照抗体(红色)作对比。

High Content Screening

High Content Screening

High content analysis of HeLa cells exposed to varying concentrations of staurosporine for 3hr. With increasing concentrations of staurosporine, a significant decrease (~2.5 fold) in phospho-MAPKAPK-2 signal as compared to the untreated control was observed. When using phospho-MAPKAPK-2 as a measurement, the IC50 of this compound was 92.5 mM. Data were generated on the Acumen HCS platform using Anti-Rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate).高通量分析HeLa细胞,该细胞暴露于不同浓度的十字孢碱3个小时。与未处理的对照组相比较,随着十字孢碱浓度的增加phospho-MAPKAPK-2信号显著降低(约2.5倍)。当采用 phospho-MAPKAPK-2检测时,IC50为92.5mM。数据时以Acumen ® HCS为平台生成的,所用抗体为Anti-Rabbit IgG (H+L), F(ab’)2 Fragment (Alexa Fluor ® 488 Conjugate)。

Applications

This product has been optimized for use as a secondary antibody in immunofluorescent applications.

Directions for Use

The optimal dilution of the secondary antibody should be determined for each application. However, a final dilution of 1:1000 should yield acceptable results for most immunofluorescent applications.

Background

Fluorescent anti-species IgG conjugates are ideal for flow cytometry and immunofluorescence. Cell Signaling Technology’s strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.

荧光抗IgG结合物适用于流式细胞术和免疫荧光。CST公司的严格的质控程序保证了每一个结合物具有最佳的特异性和荧光强度。

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

Acumen is a registered trademarks of TTP Labtech.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

The Alexa Fluor dye antibody conjugates in this product are sold under license from Life Technologies Corporation, for research use only for immunocytometry, immunohistochemistry, high content screening (HCS) analysis, or flow cytometry applications.

The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer (whether the buyer is an academic or for-profit entity), for Immunocytochemistry, high content screening (HCS) analysis, or flow cytometry applications. The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) resale, whether or not such product or its components are resold for use in research; or for any other commercial purpose. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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